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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Druckformat: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.v5.i1.30
pages 27-47

A Reference Technique to Compare the Antimicrobial Properties of Atmospheric Pressure Plasma Sources

Miriam S. Mann
Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany; Fraunhofer Institute for Cell Therapy and Immunology IZI, 18057 Rostock, Germany
Uta Schnabel
Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany
Thomas Weihe
Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany
Klaus-Dieter Weltmann
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Thomas von Woedtke
Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany


Medical devices need to submit stringent controls to gain a high quality standard. Therefore, international comparability has to be ensured between the laboratories or the producing industries via globally standardized assays. For medically used plasma sources, we developed a microbial assay that allows comparison of plasma sources with different designs. In the present case, test sources are an atmospheric pressure plasma jet (kINPen MED, neoplas tools GmbH, Greifswald, Germany) and a surface dielectric barrier discharge. Beforehand, both sources were tested for their antimicrobial antimicrobial effectiveness. We normalized the data by the treated surface area and thereby defined a smallest treated unit area. Subsequently, the values were used to obtain inactivation kinetics for comparison. By normalizing the data obtained from treatment by different plasma sources we were able to compare plasma sources, independent of the treatment time. To produce an extensive overview, the assay was carried out with Escherichia coli K12 (gram-negative), Staphylococcus aureus (gram-positive), and Candida albicans (yeast). The microorganisms revealed different inactivation kinetics, which offered for all tested objects the highest test stability in the stationary phase. Thus, to gain the most conclusive test data, microorganisms in the stationary phase are recommended for the assay.

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