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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Druckformat: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2019028659
pages 379-401

Comparative Study between Direct and Indirect Treatment with Cold Atmospheric Plasma on In Vitro and In Vivo Models of Wound Healing

Constance Duchesne
LPP, Ecole Polytechnique, UPMC, Université Paris Sud 11, CNRS, Palaiseau, France; Institut de Recherche Biomédicale des Armées, INSERM UMRS-MD 1197, Centre de Transfusion Sanguine des Armées, 92141 Clamart, France
Nadira Frescaline
LPP, Ecole Polytechnique, UPMC, Université Paris Sud 11, CNRS, Palaiseau, France; Institut de Recherche Biomédicale des Armées, INSERM UMRS-MD 1197, Centre de Transfusion Sanguine des Armées, 92141 Clamart, France
Jean-Jacques Lataillade
Institut de Recherche Biomédicale des Armées, INSERM UMRS-MD 1197, Centre de Transfusion Sanguine des Armées, 92141 Clamart, France
Antoine Rousseau
LPP, Ecole Polytechnique, UPMC, Université Paris Sud 11, CNRS, Palaiseau, France


Cold-atmospheric plasma (CAP) produces a mixture of molecular, ionic, and radical species as well as electric field visible and ultraviolet lights. Biological effects of CAP and its therapeutic potential have been studied in disciplines such as dermatology, oncology, and dentistry. This study investigates both in vitro and in vivo effects of direct and indirect plasma treatment and their influences on wound healing. The effect of plasma treatment on cellular viability, migration, and proliferation are studied using keratinocytes, fibroblasts, and endothelial cells. Plasma is generated in a helium jet using an alternating-current 50-Hz power supply at 32 kV and 90 mW. Results show that 1-min direct CAP treatment stimulates skin cell migration; however, cellular proliferation remains unchanged. Treatment > 3 min leads to cell death. Using the same treatment parameters, notably exposure time, indirect treatment using a plasma-activated medium fails to stimulate cellular migration. A murine model of full-thickness excisional wound healing is used to study the effect of CAP on wound closure. In vivo studies demonstrate that both direct and indirect treatment do not affect acute wound closure in mice. Taken together, these results suggest that direct plasma treatment with homemade plasma devices has the potential to positively influence wound healing, but optimum parameters and suitable wound models must be identified and validated.


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