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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Druckformat: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v4.i3.30
pages 201-212

Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion

Tetyana V Shimanskaya
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Yulia V. Goshovska
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Olena M. Semenykhina
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Vadim F. Sagach
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRAKT

The present study was aimed at investigating the effects of sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, on the changes in the rat heart functioning, its reserve ability under functional load and ischemia-reperfusion (I/R) injury. Isolated hearts were Langendorf-perfused and subjected to 20-minute non-flow ischemia followed by 40-minute reperfusion. The heart function was assessed by measuring the LVDP, dP/dt, coronary flow, and the heart rate. NaHS ("Sigma", 7.4 mg/kg, i. p) was injected 30 min before experiment. The opening of mitochondria permeability transition pore (mPTP) was estimated from a release of a stable mitochondrial factor (λ = 250nm) into the coronary flow. It has been shown that NaHS increases functional reserves of the heart, and the heart mitochondrial membrane potential, but it does not change UCP3 gene expression. NaHS-induced post-ischemic restoration of the heart function is more pronounced, than in control series, due to reduced mitochondrial permeability transition pore formation. Thus, hydrogen sulfide donor provides cardioprotective effect by inhibition of mPTP opening.


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