Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Druckformat: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v1.i4.40
pages 335-344

Effects of Genipin at NO synthesis and Ischemia-Reperfusion Induced Oxidative Stress in Old Rat Hearts

Vadim F. Sagach
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Yulia V. Goshovska
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Yulia P. Korkach
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Tetyana V Shimanskaya
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Anatoliy V. Kotsuruba
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRAKT

Genipin is aglycone of genipiside, one of the active compounds of Gardenia gasminoides Ellis. The gardenia fruit extract has been used in traditional Chinese medicine to relieve the symptoms of type 2 diabetes which is accompanied by extensive oxidative stress and endothelial dysfunction of NO production. Besides, genipin was shown to inhibit UCP-dependent proton leak through the inner mitochondrial membrane, which leads to the increased mitochondrial potential and ATP production. We have studied the effects of genipin at ischemia/reperfusion-induced oxidative stress and activity of NOS isoenzymes using Langendorf perfused old rat heart model. Ischemia-reperfusion is a well known oxidative agent, which induces significant increase in superoxide radical, hydrogen peroxide and hydroxyl radical content. Genipin administration in a dose of 10−5 M for 15 minutes before the prolonged ischemia exerted powerful antiradical and antilipoperoxidative effects. Heart ischemia-reperfusion was accompanied by generation of peroxinitrite and by nitrozative stress. We have demonstrated the inhibitory impact of genipin on iNOS expression, which is possibly, occurs via preoteinkinase A inhibition and via stabilization of I-κB-NF-κB complex. Genipin stimulated cNOS activity perhaps by activating PI3K/Akt signaling pathway. Although, post-ischemic recovery of cardiodinamic parameters of old rat hearts was depressed due to the "switch of" of the NO production by inducible NOS, which is important in early period of reperfusion. Thus, we conclude that genipin is a powerful antioxidant and it processes an insulin-like activity due to its ability to manage NO production at the level of intracellular signaling cascades.


Articles with similar content:

Long-term Exercise Training Improves Heart Function in Rats by NO-Dependent Decrease in Mitochondrial Pore Sensitivity to Calcium
International Journal of Physiology and Pathophysiology, Vol.7, 2016, issue 1
Snizhana V. Chorna, Nataliya A. Strutynska, Viktor E. Dosenko, Galyna L. Vavilova, Vadim F. Sagach, Anatoliy V. Kotsuruba, Olena M. Semenykhina, Sergiy O. Talanov
Changes in Myocardium Metabolism in Ischemia-Reperfusion and Activation of ATP-Sensitive Potassium Channels
International Journal of Physiology and Pathophysiology, Vol.3, 2012, issue 3
Olexiy O. Moibenko, Roman A. Rovenets, Ruslan B. Strutynskyi, Anatoliy V. Kotsuruba, Anatolii P. Neshcheret
Biochemical Mechanisms of Cardioprotective Action of the Drug Product Flocalin, ATP-Sensitive K+ Channel Opener, in Myocardial Ischemia −Reperfusion
International Journal of Physiology and Pathophysiology, Vol.5, 2014, issue 3
Nataliya A. Strutynska, Roman A. Rovenets, Ruslan B. Strutynskyi, Vadim F. Sagach, Anatoliy V. Kotsuruba, Oleksiy O. Moibenko, Yuriy L. Yagupolskii
Inhibition of Mitochondrial Hydrogen Sulfide Production Worsens Heart Contractility and Enhances Sensitivity of Mitochondrial Permeability Transition Pore to Calcium
International Journal of Physiology and Pathophysiology, Vol.9, 2018, issue 2
Nataliya A. Strutynska, Alina Yu. Luchkova, Vadim F. Sagach, Yuliya V. Hoshovs'ka, Raisa A. Fedichkina
Changes in UCP2 and UCP3 Genes Expression, in Heart Function and Oxygen Cost of Myocardial Work under Aging and Ischemia-Reperfusion
International Journal of Physiology and Pathophysiology, Vol.1, 2010, issue 3
Olexandr O. Lisovyi, Yulia V. Goshovska, Vadim F. Sagach, Tetyana V Shimanskaya