Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.950
pages 462-463

Immunomodulating Activity of Bjerkandera sp.

Mark M. Shamtsyan
Department of Technology of Microbiological Synthesis, St. Petersburg State Institute of Technology (Technical University), 26 Moscovsky Pr., 198013 St. Petersburg, Russia
Valentina G. Konusova
Laboratory of Immunopharmacology, State Research Institute of Highly Pure Biopreparations, 7 Pudozhskaya St., 197110 St. Petersburg, Russia
Natalia V. Pigareva
Laboratory of Immunopharmacology, State Research Institute of Highly Pure Biopreparations, 7 Pudozhskaya St., 197110 St. Petersburg, Russia
Alexander M. Goloschev
Department of Technology of Microbiological Synthesis, St. Petersburg State Institute of Technology (Technical University), 26 Moscovsky Pr., 198013 St. Petersburg, Russia
Arina V. Korchmaryova
Department of Technology of Microbiological Synthesis, St. Petersburg State Institute of Technology (Technical University), 26 Moscovsky Pr., 198013 St. Petersburg, Russia

ABSTRAKT

Medicinal mushrooms are widely used now as traditional medicinal ingredients for the treatment of various diseases and related health problems. Our studies were devoted to the biological activity of submerged mycelium of wood-rooting mushroom Bjerkandera sp. (Hapalopilaceae, Polyporales). Fruit bodies of the mushroom were collected in the forest of the Leningrad region of Russia and introduced into culture. Mycelium was cultivated on the semi-synthetic media containing glucose as a carbon source and peptone as a source of nitrogen.
Immunomodulating activity of aqueous extracts from fruit bodies and submerged mycelium was preliminarily studied in a test for generation of reactive forms of oxygen by the cells of human peripheral blood, using the method of luminal-dependent chemiluminescence. The test was selected on the basis of the supposition about stimulating effect of extracts on phagocytes, because it is known that compounds of a polysaccharide nature boost phagocytosis. Obtained results demonstrated pronounced activity of the extracts from fruit bodies. Mycelia extracts also possess significant activity, but the level of chemiluminescence detected for mycelium was lower than for fruit bodies.
The mitogenic activity of mycelium extracts was studied in the reaction of blast transformation of spleen and thymus cells of CBA strain mice. Fruit body extracts demonstrated high proliferation activity on spleen cells, whereas mycelium extracts were active only in relatively high concentrations. Thymus cells were not influenced by fruit body extracts or by mycelium. From the obtained results we can conclude that the studied preparations from fruit bodies and submerged mycelium of Bjerkandera sp. generally stimulate B-lymphocytes and macrophages, causing a significantly lower influence on the functional activity of T-lymphocytes.
The ability of extracts to participate in the synthesis by human blood cells of immunoregulating proteins—cytokines, particularly interleukine-1β and interleukine-8, a key mediator of inflammatory and immune responses of an organism—was studied. The results display a stimulating effect on both proinflammatory cytokines production by peripheral blood cells. On the contrary, there was no influence of extracts detected for the production of interleukine-2.
Interesting results, which were not detected for the extracts of other Basidiomycetes, were obtained during the studies of the influence of extracts of submerged mycelium of Bjerkandera sp. on the proliferation of spleen and thymus cells, preliminarily stimulated by concanavaline A. It appears that the addition of high concentrations of extracts was suppressing the proliferation of overactivated T and B cells. The resembling effect was detected during the studies of the influence of preparations on the production of interleukine-2 by concanavaline A stimulated spleen cells. Low concentrations of extract (less than 6 mg/mL) did not influence the activity of stimulated cells, whereas higher concentrations of extracts sharply decreased the levels of interleukine-2.
The detected ability of extracts of Bjerkandera sp. to suppress the activity of overstimulated immuno-competent cells can be useful in the development of preparations for treatment of various disorders of the immune system, such as allergies and autoimmune diseases.


Articles with similar content:

Specificity of the Immunomodulating Activity of Sasa veitchii (Japanese Folk Medicine Kumazasa) to Fungal Polysaccharides
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Wataru Sato, Naohito Ohno, Ken-ichi Ishibashi
Comparative Studies on the Influence of Higher Basidiomycetes Polysaccharide Fractions on Reactive Oxidizing Species Production
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 2
Tajalli Keshavarz, Gopalkrishna Lakkireddy, Christopher Bucke
Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi
Production of Ligninolytic Enzymes by Oyster Mushroom Pleurotus ostreatus (Jacq.:Fr.) P. Kumm. Under Different Nutritional Conditions
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Nona A. Mikiashvili
Effect of the Carbon Source and Inoculum Preparation Method on Laccase and Manganese Peroxidase Production in Submerged Cultivation by the Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
George G. Songulashvili, Yitzhak Hadar, Vladimir I. Elisashvili, Eviatar D. Nevo