Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i5.20
pages 427-434

Isolation and Characterization of Lectins Formed by Cerrena unicolor (Higher Basidiomycetes) in Solid-State Fermentation of Sorghum and Wheat Straw

Elene Davitashvili
Faculty of Natural Science, Iv. Javakhishvili State University, Tbilisi, Georgia
Ekaterine Kapanadze
Faculty of Exact and Natural Sciences, Department of Biology, I. Javakhishvili Tbilisi State University, 0143 Tbilisi, Georgia
Eva Kachlishvili
The Agricultural University of Georgia, University Campus at Digomi, Tbilisi, Georgia
Nona A. Mikiashvili
Institute of Evolution, University of Haifa, Israel; Iv. Javakhishvili State University of Tbilisi, Department of Biochemistry and Biotechnology, Georgia; Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, USA
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia

ABSTRAKT

The capability of Cerrena unicolor to produce fruiting bodies and lectins was studied in solid-state fermentation of a sorghum and wheat straw mixture. The first primordia appeared on day 48 and reached 6−10 mm; however, no formation of fruiting bodies occurred and these rudiments were harvested on day 55. The protein content in the rudiment extracts was significantly higher, whereas the specific hemagglutinating activity (HA) was sixfold lower as compared with those in extracts from mycelial biomass. Moreover, the specific HA of the 80-day mycelium increased to 16,667 U/mg, exceeding by sixfold that of 55-day-old mycelium. Four protein fractions (160, 105, 67, and 8 kDa) were detected by gel-chromatography of mycelial biomass crude extract; the highest specific HA was revealed in fraction III (26336 U HA/mg). Among sugars tested, galactose was the most potent inhibitor of HA of all protein fractions, with minimal inhibition concentrations of 0.095−0.780 mM. The galactose-specific lectins isolated from the fractions II and III by affinity chromatography ranged from 15 to 116 kDa and differed with kinetic parameters.


Articles with similar content:

Comparative Study of Lectin Activity of Higher Basidiomycetes
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 1
Solomon P. Wasser, Nona A. Mikiashvili, Vladimir I. Elisashvili, Eviatar D. Nevo
Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii (Higher Basidiomycetes) and its Composition
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Shin-Yi Lin, Jeng-Leun Mau, Ling-Yi Huang, Kung-Jui Ho, Chih-Hung Liang
Immunomodulation Activity of a Polysaccharide Fraction of a Culinary-Medicinal Mushroom, Pleurotus citrinopileatus Singer (Agaricomycetideae), in Vitro
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 3
Ken-ichiro Minato
Comparative Study of the Hemagglutinating Activity of Lectins Isolated from Different Developmental Stages of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kumm. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Eka Metreveli, Vladimir I. Elisashvili
Antitumor Properties of Submerged Cultivated Biomass and Extracts of Medicinal Mushrooms of Genus Hypsizygus Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
Anatoliy I. Usov, Elena B. Isakova, Anastasia V. Avtonomova, Larissa M. Krasnopolskaya, Maria I. Leontieva, Igor V. Belitsky, Vladimir M. Bukhman