Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.211 5-jähriger Impact-Faktor: 1.394 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v7.i4.60
pages 565-572

Molecular Identification of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data

S. K. Singh
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India
R. D. Rai
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP
Shwet Kamal
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India

ABSTRAKT

Analysis of genetic relationship of important Aphyllophorales germplasm accessions of the National Research Centre for Mushroom, Solan, India, was conducted using RAPD and ITS rDNA sequences. RAPD profiles exhibited significant variations among Trametes versicolor, Tyromyces chioneus, and Ganoderma species. The resulting RAPD-based phylogram grouped Ganoderma spp. accessions into four clades. Phylogeny inferred from ITS rDNA sequence distinguished Trametes versicolor, Tyromyces chioneus, and Ganoderma spp. at the generic level due to different lengths and variations of the 5.8S rDNA conserved region. At the intrageneric level, Ganoderma accessions were divided into two distinct groups based on nucleotide substitution in ITS-1 and ITS-2 regions. It is presumed that some of the Ganoderma sp. samples might be G. lucidum. Single nucleotide substitutions turned out to be more useful for genetic cataloguing of isolates within a species than sequence alignment using NCBI databse.


Articles with similar content:

New Taxa for the Flora of Ukraine, in the Context of Modern Approaches to Taxonomy of Cyanoprokaryota/Cyanobacteria
International Journal on Algae, Vol.18, 2016, issue 4
U. Karsten, T. I. Mikhailyuk, K. Glaser , O. N. Vinogradova
Molecular Identification and Characterization of the Edible and Medicinal Morchellaceae Germplasm Collection of "Mulch Morels"
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 4
Pavel Havranek, Vladan Ondrej, Pavla Nemcova, Miloslav Kitner
Genetic Variability of the Medicinal Tinder Bracket Polypore, Fomes fomentarius (Agaricomycetes), from the Asian Part of Russia
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 6
Susanna M. Badalyan, Victor A. Mukhin, Elena V. Zhuykova
Characterization of Somatic Hybrids with Compatible and Incompatible Species by Protoplast Fusion in Genera Pleurotus (Fr.) P. Karst. and Ganoderma P. Karst. by RAPD-PCR Analysis
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 2
Beom Gi Kim, Kang Hyo Lee, Young Bok Yoo
On the Taxonomy and Nomenclature of Some Terrestrial Taxa of Plectonema s. I. (Cyanophyceae). 1. The Case of Plectonema edaphicum
International Journal on Algae, Vol.20, 2018, issue 3
T. I. Mikhailyuk, O. N. Vinogradova