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International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018029002
pages 47-57

Developing a Novel Two-Stage Process for Carotenoid Production by Cordyceps militaris (Ascomycetes)

Qianwang Zheng
Institute of Food Biotechnology and College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, China
Tao Wei
Institute of Food Biotechnology and College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, China
Yin Lin
Institute of Food Biotechnology and College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China
Zhiwei Ye
College of Food Science and Institute of Food Biotechnology, South China Agriculture University, Guangzhou 510642, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China
Jun-Fang Lin
College of Food Science, South China Agricultural University
Li-qiong Guo
College of Food Science and Institute of Food Biotechnology, South China Agriculture University, Guangzhou 510642, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China; Alchemy Biotechnology Co., Ltd. of Guangzhou City, Guangzhou 510760, China
Fan Yun
Alchemy Biotechnology Co., Ltd. of Guangzhou City, Guangzhou 510760, China
Linzhi Kang
Alchemy Biotechnology Co., Ltd. of Guangzhou City, Guangzhou 510760, China

ABSTRAKT

Natural carotenoids are attracting increasing interest, but their widespread use is limited because of poor production. Cordyceps militaris, a traditional Chinese mushroom, contains a large amount of carotenoids, and this study aimed to increase carotenoid production by C. militaris by optimizing a liquid-state cultivation system. We developed and optimized a novel 2-stage process, including cultivation under shaking in darkness and under static irradiation on a flat panel, using response surface methodology, and we compared this process to common shake-flask cultivation. In addition, we examined the effects of different inducers (chitosan, peanut oil, tomato juice, yeast, and metal ions) on carotenoid production. Results showed that under optimal conditions (4 days of shaking in darkness, 10 days of static irradiation with a 100-mL flat panel volume), a maximum of 1217.5 ± 115.9 μg/g carotenoids were produced; only 662.9 μg/g were produced by common shake-flask cultivation. Only a large amount of chitosan (8 mg) could significantly increase carotenoid content; some of the other inducers showed inhibitory effects. This study demonstrated that this 2-stage process could effectively increase the natural carotenoid content in C. militaris, making it a potential source for commercial exploitation.