Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i10.80
pages 987-996

Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes)

Xiulian Yin
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China
Qinghong You
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China; Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals & Oils, Nanjing University of Finance and Economics, Nanjing, China
Xinghai Zhou
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China

ABSTRAKT

Agaricus bisporus polysaccharides (ABP) were extracted by complex enzyme-assisted extraction methodology. The following were optimal conditions for the extraction of crude ABP: complex enzyme amount, 2.2%; temperature, 62°C; time, 3 h; and pH, 4. Under these conditions, the experimental yield of crude ABP was 6.87%. The crude ABP was purified by diethylaminoethyl-cellulose 52 chromatography and Sephadex G-100 chromatography, and one fraction−namely, ABP-1−was produced. The ABP-1 contained 93.67% carbohydrate, 1.46% protein, and 0.62% uronic acid. The constituent monosaccharides were predominantly glucose, galactose, mannose, and xylose. The antioxidant activities of ABP-1 were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, its ferric-reducing activity power, and the reducing power assay. At a concentration of 1.2 mg/mL, ABP-1 seemed to possess good free radical scavenging activity, with a scavenging value of about 56%. The results indicate that ABP-1 has good antioxidant activity.


Articles with similar content:

Chemical Composition and Medicinal Value of the New Ganoderma tsugae var. jannieae CBS-120304 Medicinal Higher Basidiomycete Mushroom
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 8
Solomon P. Wasser, Lital E. Sharvit, Mikheil D. Asatiani, Gayane S. Barseghyan, Jannie Siew Lee Chan, Beny Trabelcy
Comparison of the Chemical Composition and Bioactive Components of Fruiting Bodies and Submerged Cultured Mycelia of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 9
Huawei Zeng, Menghua Ma, Mei Ying-jie, Jianfan Sund, Wenfeng Wang, Guohua Xu
Isolation and purification of sulfated anticoagulant compound from fermented Irish moss (Chondrus ocellatus)
International Journal on Algae, Vol.10, 2008, issue 4
M. De Zoysa, J. Lee, W. A. Pushpamali, Ch. Nikapitiya
Antioxidant and Gene Protective Effects of Medicinal Mushrooms Inonotus obliquus (Pers.:Fr.) Pilat and Phellinus robustus (P. Karst.) Bourd. et Galz.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Victor T. Bilay, Nina A. Bisko, Victor V. Shcherba, Nadezda Yu. Mitropolskaya
Chemical Characterization and Structure of Exopolysaccharides from Submerged Culture of New Medicinal Mushroom from China, Phellinus mori (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Feng Peng, Bao-Kai Cui, Chunlei Cao