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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i2.110
pages 185-192

The Influence of Different Submerged Cultivation Conditions on Mycelial Biomass and Protease Production by Lentinus citrinus Walleyn et Rammeloo DPUA 1535 (Agaricomycetideae)

Larissa de Souza Kirsch
Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil
Ana Carolina dos Santos Pinto
Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil
Tatiana Souza Porto
Laboratory of Immunopathology Keizo Asami (LIKA), UFRPE, Recife, PE, Brazil
Ana Lucia Figueiredo Porto
Department of Morphology and Animal Physiology, UFRPE, Recife, PE, Brazil
Maria Francisca Simas Teixeira
Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil

ABSTRAKT

The influence of different carbon and nitrogen sources, pH of the culture medium, and temperature and period of cultivation on mycelial biomass production and protease activity by Lentinus citrinus DPUA 1535 were investigated in submerged culture. A 25 full factorial design with three central points was employed, and the results showed that at a significance level of 95% only nitrogen source and temperature were statistically significant for mycelial biomass production. On the other hand, for protease activity all factors and some interactions were significant, and the temperature and nitrogen source had the most significant effect. The best condition for mycelial biomass production (5.76 mg mL−1) and protease activity (32.3 U mL−1) was obtained in medium formulated with 0.5% soluble starch, 0.2% gelatin, pH 7.0, 25°C, in 5 days.


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