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International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017024487
pages 1029-1040

Identification of Reference Genes and Analysis of Heat Shock Protein Gene Expression in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, after Exposure to Heat Stress

Yong-Nan Liu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Xiao-Xiao Lu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Ang Ren
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Liang Shi
College of Life Sciences, Nanjing Agricultural University; Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, Nanjing 210095, Jiangsu, People's Republic of China
Ai-Liang Jiang
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Han-Shou Yu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Mingwen Zhao
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China

ABSTRAKT

Ganoderma lucidum has been considered an emerging model species for studying how environmental factors regulate the growth, development, and secondary metabolism of Basidiomycetes. Heat stress, which is one of the most important environmental abiotic stresses, seriously affects the growth, development, and yield of microorganisms. Understanding the response to heat stress has gradually become a hotspot in microorganism research. But suitable reference genes for expression analysis under heat stress have not been reported in G. lucidum. In this study, we systematically identified 11 candidate reference genes that were measured using reverse transcriptase quantitative polymerase chain reaction, and the gene expression stability was analyzed under heat stress conditions using geNorm and NormFinder. The results show that 5 reference genes−CYP and TIF, followed by UCE2, ACTIN, and UBQ1−are the most stable genes under our experimental conditions. Moreover, the relative expression levels of 3 heat stress response genes (hsp17.4, hsp70, and hsp90) were analyzed under heat stress conditions with different normalization strategies. The results show that use of a gene with unstable expression (SAND) as the reference gene leads to biased data and misinterpretations of the target gene expression level under heat stress.