Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i4.70
pages 409-418

Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae)

Jozica Habijanic
National Institute of Chemistry, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Mirjan Svagelj
Department of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana
Marin BEROVIC
Prof.Dr.Bioche.Eng.
Bojana Boh
Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia
Branka Wraber
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia

ABSTRAKT

Original strains of Ganoderma lucidum (MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid-state cultivation. In G. lucidum after 14 days of submerged fed-batch cultivation, up to 17.0 g L−1 dry fungal biomass was produced. Extracellular (1.7 g L−1) and intracellular (0.45 g L−1) polysaccharide fractions were isolated, while in 18 days of solid-state cultivation, 5.77 mg/g of extracellular and 1.45 mg/g of intracellular polysaccharide were produced at the end of the cultivation period. In 28 days of submerged cultivation of G. frondosa, 3.65 mg L−1 of extracellular and 1.30 mg L−1 of intracellular polysaccharide were produced. In 38 days of solid-state cultivation, 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, gel, and affinity chromatography. The isolated polysaccharides were mainly β-D-glucans. Immunostimulatory effects of isolates were tested for induction of cytokine (TNF-α and IFN-γ) synthesis in human peripheral blood mononuclear cells (PBMC) isolated from buffy coat.


Articles with similar content:

Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian Habitats: Cultivation, Isolation, and Testing of Active Compounds
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 1
Irena Zore, Branka Wraber, Jozica Habijanic, Damjan Hodzar, Bojana Boh, Marin BEROVIC
Immunomodulating Activity of Bjerkandera sp.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Mark M. Shamtsyan, Valentina G. Konusova, Alexander M. Goloschev, Natalia V. Pigareva, Arina V. Korchmaryova
Anti-Inflammation and Lipogenic Inhibition of Taiwanofungus salmonea Mycelium and Grifola frondosa Fruiting Body
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 7
Jeng-Leun Mau, Yi-Chi Yang, Eric Icheng Lai, Rao-Chi Chien
Immunomodulating Activities of Cultivated Maitake Medicinal Mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes) on Peripheral Blood Mononuclear Cells
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Sasa Simcic, Branka Wraber, Andrej Gregori, Mirjan Svagelj, Bojana Boh, Marin BEROVIC
Production of Polysaccharide by Culinary-Medicinal Mushroom Agaricus brasiliensis S. Wasser et al. LPB 03 (Agaricomycetideae) in Submerged Fermentation and Its Antitumor Effect
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 1
Aurora L. Pedroso, Carlos Ricardo Soccol, Leifa Fan, Renato Rau, Sandro Germano, Ashok Pandey, Andrea Thomaz Soccol