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International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031138
pages 657-669

Radical Scavenging and Antiproliferative Effects of Cordycepin-Rich Ethanol Extract from Brown Rice−Cultivated Cordyceps militaris (Ascomycetes) Mycelium on Breast Cancer Cell Lines

Han-Chiang Wu
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Shou-Tung Chen
Endoscopy and Oncoplastic Breast Surgery Center, Changhua Christian Hospital, Changhua 500, Taiwan
Jui-Chih Chang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan; Vascular and Genomic Center, Changhua Christian Hospital, Changhua 500, Taiwan
Ting-Yung Hsu
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Chi-Chia Cheng
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Huei-Shin Chang
Vascular and Genomic Center, Changhua Christian Hospital, Changhua 500, Taiwan
Chin-San Liu
Vascular and Genomic Center, Changhua Christian Hospital, Changhua 500, Taiwan; Department of Neurology, Changhua Christian Hospital, Changhua 500, Taiwan; School of Chinese Medicine, Graduate Institute of Integrated Medicine, Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 40447, Taiwan
Yao-Chung Wu
Department of General Surgery, Changhua Christian Hospital, Changhua 500, Taiwan
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan


The yield and efficacy of bioactive compounds from Cordyceps militaris fruiting bodies and its fermented grains usually vary with the strain used. In this study, we compared the antiproliferative, apoptotic, and antioxidative properties of ethanolic extracts of fruiting bodies and solid-stated fermented rice (FRE) from two wild-type strains of C. militaris applied to human breast cancer cell lines. We observed that FRE of the Zhangzhou strain (FRE-Z) produced a high level of cordycepin and exhibited comprehensive in vitro antioxidant activity against the oxidation of 2,2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radicals and low-density lipoprotein. Only FRE-Z exhibited dose-dependent inhibition of cell proliferation in MCF-7 (0.7 mg/mL) and MDA-MB-231 cells (1 mg/mL) after culturing for 24 h. The antiproliferative effects of FRE-Z were associated with an early stage of apoptosis induction at 4 h of treatment with 0.5 mg/mL FRE-Z in MCF-7 cells. The antiproliferative effect was determined to occur through p53 activation but not through the release of mitochondrial apoptosis-inducing factor or caspase-9 activation for an initial culture period of 16 h. In addition to a transient increase in cellular antioxidant enzyme, Cu/Zn superoxide dismutase was identified in MCF-7 cells after 2 h of treatment with FRE-Z. Therefore, FRE-Z, which exhibits various dose− and exposure time−dependent activities, has potential application in breast cancer chemoprevention.


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