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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
Indexed in
Fractionation and Characterization of Water-Soluble Polysaccharides from Culinary-Medicinal Mushroom, Agaricus blаzеi Murrill (Agaricomycetideae) Fruit Body
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ABSTRAKT
Three groups of water-soluble polysaccharides, AB-I, AB-II-b, and AB-III-b, were obtained from Agaricus blazei fruit body by hot-water extraction, ethanol precipitation, DEAE-cellulose chroniatography, and Sephadex G-100 gel filtration. HPLC examination showed that they were purified into pure preparations. The molecular weight, monosaccharide constituent, and glucosidic bond of the polysaccharides were tested by employing Sepharose CL-4B, GC, and IR. The results were as follows: molecular weight: AB-I, 21 ґ 104, AB-II-b, 34 ґ 106, AB-III-b, 6 ґ 104; monosaccharide composition: AB-I, Gal:Man:Glc (1.43:2.02:5.57), AB-II-b, Rha:Gal:Man:Glc (1.00:1.26:2.75:4.26), AB-III-b, Rha:Xyl:Gal:Man:Glc (1.49:0.37:0.84:1.86:4.05). IR examination showed that the glucosidic bond of the three polysaccharides were b-pyran type. The polysaccharides contained a protein constituent.