Erscheint 12 Ausgaben pro Jahr
ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344
Indexed in
Study of Hypsizygus marmoreus (Peck) Bigel. and Grifola frondosa (Dicks.: Fr.) S.F. Gray: Cultural-Morphological Peculiarities, Growth Characteristics, Qualitative Enzymatic Activity, and Resistance to Fungal Pest Contamination
ABSTRAKT
Hypsizygus marmoreus (HM) and Grifola frondosa (GF) are two highly praised culinary-medicinal mushrooms with good taste, texture, and fragrance. They possess health-enhancing properties and good commercial potential worldwide. We aimed to develop biotechnologically viable cultivation methods for these mushrooms, and to reveal the taxonomic significance of several growth and macro- and micromorphological characteristics of vegetative mycelia. Among the HM strains, we have documented vegetative mycelium with incrustations, networks of vegetative hyphae, some with short side protuberances, arthroconidia, some of them connected as chains, chlamydospores and intercalary chlamydospores, and clamp connections. GF structures included vegetative hyphae with clamps, anastomoses, and terminal chlamydospores. We have found that HM strains favored malt extract agar (MEA) and wort agar (WA) the most, with pH of 5.5 and 6.0. A growth coefficient of 47.3 was the highest and belonged to HM strain 571. Among GF strains, potato dextrose agar (PDA) was the most favored media at pH 4.5; GC of 58.2 was the highest and belonged to GF strain 924. In the solid-state measurement of laccase activity on guaiacol, we have found that enzyme secretion was associated with the agar media, or guaiacol, and not with the supplement, sawdust, or straw. Other enzymes demonstrated very low activity. When observing the relationship between the study mushroom mycelia and pest fungi, we have found that in all strains of the study mushrooms Aspergillus versicolor formed a border with GF and later overgrew it; HM formed a border with A. versicolor and later overgrew it; and Penicillium brevicompactum inhibited both HM and GF. Both mushrooms and pests did not form any contact with each other during these studies. The above-mentioned characteristics could be useful in culture for in vitro identification protocol of screened species during their biotechnological cultivation process.
-
Akavia E., Beharav A., Wasser S.P., Nevo E., Disposal of agro-industrial by-products by organic cultivation of the culinary and medicinal mushroom Hypsizygus marmoreus, Waste Management, 29, 5, 2009. Crossref
-
Zhang Jinjing, Chen Hui, Chen Mingjie, Ren Ang, Huang Jianchun, Wang Hong, Zhao Mingwen, Feng Zhiyong, Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus, Microbiological Research, 179, 2015. Crossref
-
Zhang Jinjing, Chen Hui, Chen Mingjie, Wang Hong, Song Xiaoxia, Feng Zhiyong, Construction and application of a gene silencing system using a dual promoter silencing vector inHypsizygus marmoreus, Journal of Basic Microbiology, 57, 1, 2017. Crossref
-
Zhang Jinjing, Ren Ang, Chen Hui, Zhao Mingwen, Shi Liang, Chen Mingjie, Wang Hong, Feng Zhiyong, Nowrousian Minou, Transcriptome Analysis and Its Application in Identifying Genes Associated with Fruiting Body Development in Basidiomycete Hypsizygus marmoreus, PLOS ONE, 10, 4, 2015. Crossref
-
Zhang Jinjing, Hao Haibo, Chen Mingjie, Wang Hong, Feng Zhiyong, Chen Hui, Hydrogen-rich water alleviates the toxicities of different stresses to mycelial growth in Hypsizygus marmoreus , AMB Express, 7, 1, 2017. Crossref
-
Chen Hui, Hai Haibo, Wang Hong, Wang Qian, Chen Mingjie, Feng Zhiyong, Ye Ming, Zhang Jinjing, Hydrogen-rich water mediates redox regulation of the antioxidant system, mycelial regeneration and fruiting body development in Hypsizygus marmoreus, Fungal Biology, 122, 5, 2018. Crossref
-
Chen Hui, Hao Haibo, Han Cancan, Wang Hong, Wang Qian, Chen Mingjie, Juan Jiaxiang, Feng Zhiyong, Zhang Jinjing, Exogenous l-ascorbic acid regulates the antioxidant system to increase the regeneration of damaged mycelia and induce the development of fruiting bodies in Hypsizygus marmoreus, Fungal Biology, 124, 6, 2020. Crossref