Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal on Algae
SJR: 0.216 SNIP: 0.322 CiteScore™: 0.4

ISSN Druckformat: 1521-9429
ISSN Online: 1940-4328

International Journal on Algae

DOI: 10.1615/InterJAlgae.v21.i4.70
pages 365-372

Fucoxanthine Content in Some Black Sea Brown Algae (Ochrophyta, Phaeophyceae)

F. P. Tkachenko
I.I. Mechnikov Odessa National University, 2 Dvorianskaya Str., Odessa 65026, Ukraine
I. P. Yakuba
I.I. Mechnikov Odessa National University 2 Dvorianskaya Str., Odessa 65026, Ukraine

ABSTRAKT

The results of the study of fucoxanthine content in the six common and abundantly grown species of brown algae: Scytosiphon lomentaria (Lyngb.) Link, Petalonia zosterifolia (Reinke) Kuntze, Punctaria latifolia Grev., Desmarestia viridis (O.F. Muller) J.V. Lamour., Ectocarpus siliculosus (Dillwyn) Lyngb. from Odessa Bay and Cystoseira barbata (Stackh.) C. Agardh from the Tiligul Estuary of the Black Sea are presented. Algae-macrophytes are a very important component of marine ecosystems. They are used in the food industry and also in agriculture as fertilizers and feed additives. The global algae industry annually processes up to 8 million tons of raw macrophyte biomass. Among processed products, polysaccharides (agar, carrageenan, fucoidan, alginates) are of great importance. They are used in the food industry, medicine, pharmaceuticals, cosmetology, etc. Biologically active substances of algae include pigments used as food coloring and anti-oxidants, as well as medicinal raw materials. Fucoxanthin is the predominant pigment of brown algae. It is used in the treatment of tumor diseases; it also has anti-inflammatory, anti-angiogenic and anti-oxidant properties, stabilizes carbohydrate metabolism and the cardiovascular system. The pigments were extracted with 96% ethanol. Separation was carried out by thin-layer chromatography on a silofol plate. The pigment concentration was determined by the optical density at the appropriate wavelength and pigment extinction coefficient in this solvent. On average, the content of fucoxanthin in the studied species of algae varies in the range of 0.55-4.11 mg/g of dry biomass. Its smallest content was observed in D. viridis, the largest -in P. latifoli. Seasonal variations in pigment content were noted: in most species, it increased from February to April. Brown algae samples were taken at two stations: in a relatively clean area and in an area with high levels of pollution near the sewage discharge of the biological treatment plant. It was shown that with an increase in the level of eutrophication of water, the content of fucoxanthin in algae decreases. P. latifolia, having the highest content of fucoxanthin among studied species of the brown algae, is a promising object of biotechnology.

REFERENZEN

  1. Brownlee I.A., Allen A., Pearson J.P., Dettmar P.W., Havler M.E., Atherton M.R., and Onsoyen E., Alginate as a source of dietary fiber, Critical Rev.. in Food Sci. and Nutr., 45(6): 497-510, 2005.

  2. Chesalin M.V., Al-Ghassani S., RyabushkoV.I., Bobko N.I., Gureeva E.V., and Nekhoroshev M.V., Fucoxanthin content and chemical composition in brown alga Nizamuddinia zanardinii (Phaeophyta) collected from Mirbat, Southern Oman (the Arabian Sea), Algologia, 27(3): 246-260, 2017. https://doi.org/10.15407/alg27.03.246.

  3. Gammone M.A., Riccioni G., and D'Orazio N., Carotenoids: potential allies of cardiovascular health?, Food & Nutr. Res, 59: 1-11, 2015.

  4. GOST 33331-2015, Seaweeds, sea grasses and products of their processing. Methods for determination of mass content of water, ash and foreign matter, Standartinform, Moscow, 2016. [Rus.].

  5. Hosokawa M., Kudo M., Kohno H., Tanaka T., and Miyashita K., Fucoxanthin induces apoptosia and enhances the antiproliferative effect of the PPARgamma ligand troglitazone, on colon cancer cells, Biochim, Biophys. Acta, 1675(1-3): 113-119, 2004.

  6. Kalugina-Gutnik A.A., Phytobenthos of the Black Sea, Nauk. Dumka Press, Kyiv, 1975. [Rus.].

  7. Kim K.N., Heo S.J., Kang S.M., Ahn G., and Jeon Y.J., Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway, Toxicol. in vitro, 24: 1648-1654, 2010.

  8. Li-JuanWang, Yong Fan, Ronald L. Parsons, Guang-Rong Hu, Pei-Yu Zhang, and Fu-Li Li, A Rapid Method for the Determination of Fucoxanthin in Diatom, Mar. Drugs, 16(1): 33, 2018.

  9. McHugh D.J., A guide to the seaweed industry, Vol. 441, FAO Fisher. Techn. Paper, Rome, 2003.

  10. Musienko M.M., Parshikova T.V., and Slavnyi P.S., Spectrophotometric methods in the practice of plant physiology, biochemistry and ecology, Phytosociocentr, Kyiv, 2001. [Ukr.].

  11. Ryabushko V.I., Musatenko L.I., Voytenko L.V., Popova E.V., and Nechoroshev M.V., Int. J. Algae, 17(1): 69-81,2015. https://doi.org/10.1615/InterJAlgae.v17.il.60.

  12. Ryabushko V.I., Prazukin A.V., Popova E.V., and Nekhoroshev M.V., Fucoxanthin of the brown algae Cystoseira barbata (Stackh.) C.Agardh from the Black Sea, J. Black Sea/Mediter. Environ, 20(2): 108-113, 2014.

  13. Ryabushko V.I., Zheleznova S.N., and Nechoroshev M.V., Effect of Nitrogen on the accumulation of fucoxanthin from diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin, Algologia. 27(1): 15-21, 2017. https://doi.org/10.15407/alg27.01.015.

  14. Takaichi S., Carotenoids in algae: Distribution, biosyntheses and functions, Mar. Drugs, 9: 1101-1118, 2011.

  15. Tkachenko F.P., Species composition of macrophyte algae of the north-western part of the Black Sea (Ukraine), Algologia, 14(3): 277-294, 2004.


Articles with similar content:

Composition and quantitative characteristics of periphyton microalgae in coastal waters near Vladivostok city (Russia)
International Journal on Algae, Vol.11, 2009, issue 3
A. Yu. Zvyagintsev, A. A. Begun, L. I. Ryabushko
Effect of Nutrients on Diversity of Algae in Marngar Lake, Meghalaya (India)
International Journal on Algae, Vol.15, 2013, issue 3
Ophilia Shylla, P. Ramanujam
Regularities of seasonal dynamics of the vertical distribution of phytoplankton in a pond
International Journal on Algae, Vol.4, 2002, issue 2
Ye. S. Kravchuk, V. I. Kolmakov, Ye. A. Ivanova, O. V. Anishchenko
Physiological and Biochemical Status of Fishes of the Fam. Percidae in Winter
Hydrobiological Journal, Vol.51, 2015, issue 1
M. V. Prychepa , O. S. Potrokhov
The effect of red light on a photosynthetic apparatus under auto- and photo-heterotrophic growth of Spirulina platensis (Nordst.) Geitl. (Cyanophyta)
International Journal on Algae, Vol.9, 2007, issue 4
Alexandr A. Sivash, T. O. Zakharova, R. N. Fomishina, Svetlana I. Los', S. N. Vasilchenko