RT Journal Article ID 0b6528d9687446ed A1 Geng, Yan A1 Zhu, Shuiling A1 Lu, Zhenming A1 Xu, Hong-Yu A1 Shi, Jin-Song A1 Xu, Zheng-Hong T1 Anti-inflammatory Activity of Mycelial Extracts from Medicinal Mushrooms JF International Journal of Medicinal Mushrooms JO IJM YR 2014 FD 2014-07-30 VO 16 IS 4 SP 319 OP 325 K1 medicinal mushrooms K1 inflammation K1 LPS K1 RAW264.7 K1 Ganoderma lucidum AB Medicinal mushrooms have been essential components of traditional Chinese herbal medicines for thousands of years, and they protect against diverse health-related conditions. The components responsible for their anti-inflammatory activity have yet to be fully studied. This study investigates the anti-inflammatory activity of n-hexane, chloroform, ethyl acetate, and methanol extracts of mycelia in submerged culture from 5 commercially available medicinal mushrooms, namely Cephalosporium sinensis, Cordyceps mortierella, Hericium erinaceus, Ganoderma lucidum, and Armillaria mellea. MTT colorimetric assay was applied to measure the cytotoxic effects of different extracts. Their anti-inflammatory activities were evaluated via inhibition against production of lipopolysaccharide (LPS)-induced nitric oxide (NO) in murine macrophage-like cell line RAW264.7 cells. Of the 20 extracts, n-hexane, chloroform, ethyl acetate, and methanol extracts from C. sinensis, C. mortierella, and G. lucidum; chloroform extracts from H. erinaceus and A. mellea; and ethyl acetate extracts from A. mellea at nontoxic concentrations (<300 μg/mL) dose-dependently inhibited LPS-induced NO production. Among them, the chloroform extract from G. lucidum was the most effective inhibitor, with the lowest half maximal inhibitory concentration (64.09 ± 6.29 μg/mL) of the LPS-induced NO production. These results indicate that extracts from medicinal mushrooms exhibited anti-inflammatory activity that might be attributable to the inhibition of NO generation and can therefore be considered a useful therapeutic and preventive approach to various inflammation-related diseases. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4c99b516238143af,0b6528d9687446ed.html