RT Journal Article
ID 10c3a2a60c6d3d0e
A1 Cong, Wei-Ran
A1 Gong, Zhen-Hua
A1 Shi, Dan-Dan
A1 Guo, Hui
A1 Zhou, Xuan-Wei
T1 Cloning of Mating-Type Gene MAT1-1 from the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes) Using TAIL-PCR Technology
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2014
FD 2014-09-12
VO 16
IS 5
SP 449
OP 461
K1 medicinal mushrooms
K1 Cordyceps militaris
K1 TAIL-PCR
K1 mating-type
K1 MAT1-1-1 gene
K1 bioinformation
AB Cordyceps militaris and Ophiocordyceps sinensis (syn. Cordyceps sinensis), 2 well-known traditional Chinese medicines, contain the same bioactive components and share a similar developmental process. In this study, one C. militaris strain preserved in our laboratory was proven to be a MAT1 mating-type strain using a polymerase chain reaction−based mating-type assay. A 5000-bp nucleotide sequence of the mating-type MAT1-1 from C. militaris was amplified by thermal asymmetric interlaced polymerase chain reaction, but genes within the mating-type MAT1-2 remain undetectable. Sequence analysis shows that the mating-type gene MAT1-1 idiomorph contains 2 genes, MAT1-1-1 and MAT1-1-2. The MAT1-1-1 gene consists of 1480-bp nucleotides that encode 456 amino acids and contain the conserved a-box domain interrupted by 2 introns; the MAT1-1-2 gene consists of 1066 nucleotides that encode 377 amino acids interrupted by one intron. The intervening distance between MAT1-1-1 and MAT1-1-2 is 778 bp. The C. militaris MAT1-1 idiomorph organization is the same as that of Cordyceps takaomontana. The MAT1-1 mating-type idiomorph of both Cordyceps species lacks the MAT1-1-3 gene, which is typically present in Pyrenomycetes. These studies provide some insights for further study of the morphological development of C. militaris and will eventually benefit the domestication of O. sinensis.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,266d4152107fca7a,10c3a2a60c6d3d0e.html