RT Journal Article ID 23fd88ea45f7f235 A1 Shimanskaya, Tetyana V A1 Goshovska, Yulia V. A1 Semenykhina, Olena M. A1 Sagach, Vadim F. T1 Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion JF International Journal of Physiology and Pathophysiology JO IJPP YR 2013 FD 2013-11-20 VO 4 IS 3 SP 201 OP 212 K1 hydrogen sulfide K1 isolated heart K1 Frank-Starling curve K1 ischemia-reperfusion K1 mitochondrial permeability transition pore K1 uncoupling proteins K1 mitochondrial membrane potential AB The present study was aimed at investigating the effects of sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, on the changes in the rat heart functioning, its reserve ability under functional load and ischemia-reperfusion (I/R) injury. Isolated hearts were Langendorf-perfused and subjected to 20-minute non-flow ischemia followed by 40-minute reperfusion. The heart function was assessed by measuring the LVDP, dP/dt, coronary flow, and the heart rate. NaHS ("Sigma", 7.4 mg/kg, i. p) was injected 30 min before experiment. The opening of mitochondria permeability transition pore (mPTP) was estimated from a release of a stable mitochondrial factor (λ = 250nm) into the coronary flow. It has been shown that NaHS increases functional reserves of the heart, and the heart mitochondrial membrane potential, but it does not change UCP3 gene expression. NaHS-induced post-ischemic restoration of the heart function is more pronounced, than in control series, due to reduced mitochondrial permeability transition pore formation. Thus, hydrogen sulfide donor provides cardioprotective effect by inhibition of mPTP opening. PB Begell House LK https://www.dl.begellhouse.com/journals/6ec4ba27650016b1,1563fd7b46186e0d,23fd88ea45f7f235.html