%0 Journal Article %A Campbell, S. M. %A Pye, Andrew %A Horton, S. %A Matthew, J. %A Helliwell, P. %A Curnow, Alison %D 2007 %I Begell House %N 4 %P 295-303 %R 10.1615/JEnvironPatholToxicolOncol.v26.i4.60 %T A Clinical Investigation to Determine the Effect of Pressure Injection on the Penetration of Topical Methyl Aminolevulinate into Nodular Basal Cell Carcinoma of the Skin %U https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,46c1c95a1b69d3bb,2eed228a57e43b6e.html %V 26 %X This investigation considered a novel method of enhancing penetration of the topical photosensitizing agent methyl aminolevulinate (MAL) into nodular basal cell carcinomas (BCCs) using an oxygen pressure injection device. Oxygen pressure injection (OPI) is a method to drive compounds into skin using pressured oxygen. The study was an observer-blinded pilot of a single application of MAL to nBCCs, with or without the use of OPI. The BCCs were then excised at different time intervals (0−180 min) and the depth of penetration of the MAL examined using microscopic fluorescence photometry to detect the production of the naturally fluorescent active photosensitiser protoporphyrin IX (PpIX). A highly selective and homogeneous distribution of MAL-induced porphyrin fluorescence was seen in all nBCC tumors studied, and showed a high lesion-to-normal-tissue ratio with very little fluorescence in the surrounding normal tissue. Although it was difficult to compare quantitatively, as individual tumors in each of the different study groups varied, a definite trend of increase in relative tumor concentration of MAL-induced PpIX was observed over time, and this was enhanced when OPI was employed. %8 2007-12-04