RT Journal Article
ID 3405d2d96ccf3e90
A1 Ko, Huey-Jiun
A1 Chen, Jyun-Han
A1 Ng, Lean-Teik
T1 Hepatoprotection of Gentiana scabra Extract and Polyphenols in Liver of Carbon Tetrachloride-Intoxicated Mice
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2011
FD 2011-11-23
VO 30
IS 3
SP 179
OP 187
K1 Gentiana scabra
K1 antioxidant
K1 hepatoprotective
K1 polyphenols
K1 CCl4
AB In traditional Chinese medicine, the rhizomes of Gentiana scabra are used to treat hepatitis, stomatitis, and inflammatory diseases. This study aimed to examine the protective effects of G. scabra aqueous extract (GS) and polyphenols in liver of CCl4-intoxicated mice. Antioxidant activities of GS were also evaluated. Results showed that GS exhibited anti-lipid peroxidation, 2,2-diphenyl-1-picrylhydrazyl, and superoxide radical scavenging activities with IC50 values of 45.84, 183.38, and 56.25 μ/mL, respectively. High-performance liquid chromatography analysis revealed that the major polyphenolic constituents of GS were kaempferol, ellagic acid, and quercetin. Daily oral administration of 500 mg/kg GS (GS500) and 1000 mg/kg GS (GS1000) significantly prevented the elevation of glutamic pyruvic transaminase and thiobarbituric acid reactive substances levels, while enhancing the levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and serum total antioxidant activity in mice with hepatoxicity. At 1000 mg/kg, GS was as effective as 100 mg/kg silymarin in reducing oxidative stress and preventing liver injury. Histopathological studies further confirmed the hepatoprotective activity of GS. Taken together, these results show that the antioxidant activities and polyphenolic compounds (kaempferol, ellagic acid, and quercetin) of GS may have contributed to its hepatoprotective activity in CCl4-intoxicated mice, and its mechanism of action could be mediated through the reduction of oxidative stress in liver tissue.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,28d5b25a1eb31fda,3405d2d96ccf3e90.html