RT Journal Article
ID 477244a02dffc73b
A1 Xin, Huijie
A1 Fang, Leilei
A1 Xie, Junbo
A1 Qi, Wuqin
A1 Niu, Yuyu
A1 Yang, Fan
A1 Cai, Dandan
A1 Zhang, Yanqing
A1 Wen, Zhiyou
T1 Identification and Quantification of Triterpenoids in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), with HPLC-MS/MS Methods
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-10-15
VO 20
IS 10
SP 919
OP 934
K1 ganoderic acid A
K1 Ganoderma lucidum
K1 HPLC-Q-TOF-MS
K1 medicinal mushrooms
K1 triterpenoids
AB Ganoderma lucidum is one of the most commonly used mushrooms in traditional Chinese medicine, with significant immunomodulatory and antitumor effects. Triterpenoids are deemed to be the main bioactive components in G. lucidum. In this study, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (MS) and high-performance liquid chromatography-MS/MS methods were used to identify and quantify, respectively, the main triterpenoids in G. lucidum obtained from different sources. The full MS scan with a daughter ion scan was used to identify a variety of potential derivatives of triterpenoids in the mushroom. Multiple reaction monitoring was performed with transitions of m/z—515.2 → 285.1 (quantifier) and 515.2 → 497.2 (qualifier)—to determine the amount of ganoderic acid A. The developed methods were successfully used to analyze 8 G. lucidum samples from the wild and from cultivated and commercial sources. The results show that the content and composition of triterpenoids differed significantly among these samples. A total of 29 triterpenoids were explicitly or tentatively identified in the wild sample, whereas 23 triterpenoids were found in the cultured sample and 17 in the commercial sample. The results of this study provide a foundation for further research on chemical constituents of, identification of metabolites in, and quality control of the G. lucidum mushroom.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e8a1fed297cf479,477244a02dffc73b.html