RT Journal Article ID 540187cb1f69514a A1 Chen, Nanguang A1 Rehman, Shakil A1 Sheppard, Colin J.R. T1 Recent Advances in Optical Microscopy Methods for Subcellular Imaging of Thick Biological Tissues JF Critical Reviews™ in Biomedical Engineering JO CRB YR 2013 FD 2014-04-14 VO 41 IS 4-5 SP 393 OP 403 K1 Microscopy; imaging depth; scattering; fluorescence; contrast AB Optical microscopy has been widely applied in cellular and subcellular imaging. Conventional light microscopes, however, have rather limited imaging depth and are limited to imaging only mechanically sectioned thin samples. Multiphoton microscopy and optical coherence microscopy are common techniques for diffraction-limited imaging beyond an imaging depth of 0.5 mm. Focal modulation microscopy is a novel method that combines confocal spatial filtering with focal modulation to reject out-of-focus backgrounds. Focal modulation microscopy has demonstrated an imaging depth comparable to those of multiphoton microscopy and optical coherence microscopy, near-real-time image acquisition, and capability with a multiple contrast mechanism. PB Begell House LK https://www.dl.begellhouse.com/journals/4b27cbfc562e21b8,4566441101015d3d,540187cb1f69514a.html