RT Journal Article ID 55ad13c81043d005 A1 Dahl, Christina A1 Ralfkiaer, Ulrik A1 Guldberg, Per T1 Methods for Detection of Subtle Mutations in Cancer Genomes JF Critical Reviews™ in Oncogenesis JO CRO YR 2006 FD 2006-08-22 VO 12 IS 1-2 SP 41 OP 74 AB With the realization that cancer is a genetic disease, detection of mutations in genomic DNA has become an important discipline in many areas of cancer research. Although the publication of the human genome sequence and the immense technological advancements have facilitated the analysis of cancer genomes, detection of mutations in tumor specimens may still be challenging and fraught with technical problems. In this review, we describe current technologies for detection of small DNA mutations, including mutation scanning techniques to search for unknown mutations, and diagnostic techniques to detect known cancer mutations. We outline the principles of the different techniques and discuss their advantages and limitations. We also discuss critical issues that must be considered before choosing methodology, including sensitivity, specificity, limit of detection, throughput and cost, quantity and quality of template DNA, available equipment, and personnel expertise. PB Begell House LK https://www.dl.begellhouse.com/journals/439f422d0783386a,153f819057b50dfa,55ad13c81043d005.html