RT Journal Article ID 5bb233401ad1cf3e A1 Yang, Kai A1 Jin, Yuezhong A1 Xing, Chen A1 Hu, Jiangning A1 Wang, Ruwei A1 Sun, Peilong T1 Chemical Characterization and In Vitro Antioxidant Activity Evaluation of Polysaccharides from the Fruiting Bodies of the Red Heart Mushroom Phellinus pini (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-05-06 VO 17 IS 3 SP 297 OP 307 K1 medicinal mushrooms K1 Phellinus pini K1 polysaccharides K1 chemical characterization K1 antioxidant activity K1 monosaccharide composition AB Phellinus pini is a precious medicinal mushroom. Three water-soluble fractions of crude polysaccharides (PP30, PP60, and PP80) were obtained from the fruiting bodies of Ph. Pini. The basic chemical characterization and in vitro antioxidant activity of these 3 polysaccharides were determined. All 3 crude polysaccharides were heteropolysaccharide complexes with a small amount of protein (1.14−2.55%) and uronic acid (2.06−4.11%). The monosaccharide composition of PP30, PP60, and PP80, as a molar ratio, was mannose (1.00):glucose (18.7):galactose (0.92), fucose (0.47):3-0-Me-Gal (0.51):mannose (1.00):glucose (7.86):galactose (1.10), and rhamnose (0.12):fucose (0.32):xylose (0.17):3-0-Me-Gal (0.26):mannose (1.00):glucose (4.79):galactose (0.53), respectively. The in vitro antioxidant activities of crude polysaccharides were evaluated by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, hydroxyl radical, and ferric-reducing antioxidant power methods. The antioxidant data obtained using these methods were in accordance with each other and decreased in the same order of PP80 > PP60 > PP30 at a concentration of 0.1−2.5 mg/mL. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b35b5ed6bb0a817,5bb233401ad1cf3e.html