%0 Journal Article %A Delben, Juliana Aparecida %A Murata, Ramiro Mendonca %A Wei, Xi %A Castro, Myrella Lessio %A Assuncao, Wirley Goncalves %A da Silva, Nelson Renato Franca Alves %A Duarte, Simone %D 2014 %I Begell House %K low-temperature plasma, oral biofilm, Candida albicans %N 1-4 %P 231-244 %R 10.1615/PlasmaMed.2014012361 %T Low-Temperature Plasma: An Effective Approach Against Candida albicans Biofilm %U https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,700f28e67d84b510,6c17828e0fce47b8.html %V 4 %X This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); −application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm. %8 2015-02-11