%0 Journal Article
%A Delben, Juliana Aparecida
%A Murata, Ramiro Mendonca
%A Wei, Xi
%A Castro, Myrella Lessio
%A Assuncao, Wirley Goncalves
%A da Silva, Nelson Renato Franca Alves
%A Duarte, Simone
%D 2014
%I Begell House
%K low-temperature plasma, oral biofilm, Candida albicans
%N 1-4
%P 231-244
%R 10.1615/PlasmaMed.2014012361
%T Low-Temperature Plasma: An Effective Approach Against Candida albicans Biofilm
%U https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,700f28e67d84b510,6c17828e0fce47b8.html
%V 4
%X This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); −application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.
%8 2015-02-11