Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.241 5-Year IF: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i1.40
pages 33-41

Andrographolide inhibits human umbilical vein endothelial cell invasion and migration by regulating MMP-2 and MMP-9 during angiogenesis

P. Pratheeshkumar
Amala Cancer Research Centre, Amala Nagar, Thrissur-680555, Kerala State, India
Girija Kuttan
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State, India

ABSTRACT

Angiogenesis, the formation of new blood vessels from pre existing blood vessels, is essential for tumour progression and metastasis. Studies using Human umbilical vein endothelial cells (HUVECs) clearly demonstrated that administration of andrographolide significantly retarded endothelial cell proliferation, migration, invasion and tube formation. Gelatin zymographic analysis showed the inhibitory effect of andrographolide on the activation of matrix metalloproteinases-MMP-2 and MMP-9. The study reveals that andrographolide treatment could inhibit the activation and nuclear translocation of p65, p50, c-Rel subunits of nuclear factor-κB, and other transcription factors such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element-binding protein in B16F-10 melanoma cells. All these results demonstrate that andrographolide inhibits in vitro angiogenesis by inhibiting MMP-2 and MMP-9 and also by regulating the nuclear translocation of transcription factors.


Articles with similar content:

13-cis-Retinoic Acid Induces Apoptosis by Modulating Caspase-3, bcl-2, and p53 Gene Expression and Regulates the Activation of Transcription Factors in B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.27, 2008, issue 3
Chaluvally Raghavan Pradeep, Chandrasekharan Guruvayoorappan, Girija Kuttan
Diallyl Disulfide Induces Caspase-Dependent Apoptosis via Mitochondria-Mediated Intrinsic Pathway in B16F-10 Melanoma Cells by Up-Regulating P53, Caspase-3 and Down-Regulating Pro-Inflammatory Cytokines and Nuclear Factor- κβ-Mediated Bcl-2 Activation
Journal of Environmental Pathology, Toxicology and Oncology, Vol.29, 2010, issue 2
P. Pratheeshkumar, Girija Kuttan, P. Thejass
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Ipobscurine, an Indole Alkaloid from Ipomoea obscura, Inhibits Tumor Cell Invasion and Experimental Metastasis by Inducing Apoptosis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 2
T. P. Hamsa, Girija Kuttan
A Polysaccharide from the Culinary-Medicinal Mushroom Pholiota nameko (Agaricomycetes) Inhibits the NF-κB Pathway in Dendritic Cells Through the TLR2 Receptor
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Guoliang Li, Lihua Huai, Yufeng Xu, Haiping Li, Ruixuan Zhu, Fengling Wang, Pei Zhao