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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Print: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v2.i3.80
pages 279-289

Effect of Anti-NaPi2b Monoclonal Antibody on Phosphate Transport in Renal Cancer Cell Line SK-RC-18

Ramziya G. Kiyamova
Department of Cell Signaling, Institute of Molecular Biology and Genetics, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine
Ganna A. Savchenko
Department of Cellular Membranology, Bogomoletz Institute of Physiology, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine
Vitalina S. Gryshkova
Department of Cell Signaling, Institute of Molecular Biology and Genetics, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine
Galina V. Ovcharenko
Department of Cell Signaling, Institute of Molecular Biology and Genetics, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine
Yaroslav A. Boychuk
Department of Cellular Membranology, Bogomoletz Institute of Physiology, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine
Oleg O. Krishtal
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Valeriy V. Filonenko
Department of Cell Signaling, Institute of Molecular Biology and Genetics, NASU, Kiev; Key State Laboratory Of Molecular And Cellular Biology, Ukraine

ABSTRACT

The sodium-dependent phosphate transporter NaPi2b (SLC34A2, NaPi-IIb, NPT2b) is involved in regulation of inorganic phosphate metabolism. Its expression was detected in a set of normal and malignant tissues predominantly on mRNA level. Here we describe NaPi2b protein expression in tubules of human kidney by using immunohistochemical analysis with anti-NaPi2b mAb. Patch-clamp technique was used to study effect of anti-NaPi2b mAb on sodium-dependent phosphate current in renal cancer cells (SK-RC-18 cell line) expressing high level of NaPi2b protein. We demonstrate that incubation of SK-RC-18 cells with anti-NaPi2b mAb decreases phosphate current in 70% cells while unspecific antibody A33 does not produce any effect. Thus, anti-NaPi2b mAb can serves as a potential specific inhibitor of NaPi2b mediated phosphate transport. NaPi2b and anti-NaPi2b mAb have a great potential for diagnosis and treatment of different pathologies related to aberrant expression and function of NaPi2b phosphate transporter.