Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i6.60
pages 583-589

Isolation and Characterization of a Glucan-Type Polysaccharide from the Red Pine Mushroom, Lactarius deliciosus (Higher Basidiomycetes)

Ana Villares
Centro para la Calidad de los Alimentos, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Campus Universitario Duques de Soria, c/ Jose Tudela s/n, 42004, Soria, Spain


A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue.

Articles with similar content:

Functional Properties of Partially Characterized Polysaccharide from the Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 10
Sarasvathy Subramaniam, Jegadeesh Raman, Umah Rani Kuppusamy, Chua Kek Heng, Vikineswary Sabaratnam
Production, Purfication, Molecular Characterization and Bioactivities of Exopolysaccharides Produced by the Wine Cap Culinary-Medicinal Mushroom, Stropharia rugosoannulata 2# (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Peixin He, Jizhong Wang, Chunping Xu, Lujing Geng
Chemical Characterization and In Vitro Antioxidant Activity Evaluation of Polysaccharides from the Fruiting Bodies of the Red Heart Mushroom Phellinus pini (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Peilong Sun, Ruwei Wang, Jiangning Hu, Chen Xing, Kai Yang, Yuezhong Jin
Structural Characterization and Immunological Activities of a Novel Water-Soluble Polysaccharide from the Fruiting Bodies of Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 9
Ting Feng, Jing-Song Zhang, Chi-Chung Lin, Wei Jia, Wenhan Wang, Hua Fan, Hai-Ying Bao
Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 4
George G. Songulashvili, Yitzhak Hadar, Vladimir I. Elisashvili, Eviatar D. Nevo