Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018025400
pages 31-46

Extrinsic and Intrinsic Apoptotic Responses Induced by Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Aqueous Extract against a Larynx Carcinoma Cell Line

Tiane C. Finimundy
Laboratory of Genomics, Proteomics and DNA Repair, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil
Gustavo Scola
Departments of Psychiatry and Pharmacology, University of Toronto, and Centre for Addiction and Mental Health, Toronto, Canada
Fernando J. Scariot
Laboratory of Applied Microbiology, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil
Aldo J. P. Dillon
Laboratory of Enzyme and Biomass, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil
Sidnei Moura
Laboratory of Biotechnology of Natural and Synthetics Products, Technology Department, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil
Sérgio Echeverrigaray
Laboratory of Applied Microbiology, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil; Cytogenes Diagnósticos Moleculares Ltda., Lajeado, Rio Grande do Sul, Brazil
João Pegas Henriques
Laboratory of Genomics, Proteomics and DNA Repair, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil
Mariana Roesch-Ely
Laboratory of Genomics, Proteomics and DNA Repair, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil

ABSTRACT

Cumulative evidence from research studies has shown that the shiitake culinary-medicinal mushroom, Lentinus edodes, is an excellent source of natural antitumor agents and is capable of inhibiting cancer cell growth. However, the cell signaling pathway that leads tumor cells to apoptosis is not well understood because many chemical compounds may be acting. This study investigated the chemopreventive effects of an L. edodes aqueous extract on human HEp-2 epithelial larynx carcinoma cells and normal human MRC-5 lung fibroblasts by identifying proliferative and apoptotic pathways. The chemical characterization of the dry powder was assessed by high-performance liquid chromatography. Antiproliferative and proapoptotic effects induced by the extract were evaluated by assessing proliferative markers, cell sorting through flow cytometry, and expression levels of apoptotic proteins with Western blotting. The results suggest that inhibition of cell proliferation was more prominent in HEp-2 than in MRC-5 cells. Cell death analysis showed the appearance of cell populations in the sub-G1 phase, with late apoptotic signal increased in a dose-dependent manner. In addition, the aqueous extract induced depolarization of mitochondria, activating the generation of intracellular reactive oxygen species in HEp-2 cells. These observations suggest that L. edodes extract may exert a chemopreventive effect, regulating mitotic induction of apoptogenic signals. These findings highlight the mushroom's pharmacological potential in cancer treatment.


Articles with similar content:

Apoptotic Effect of Extract from Medicinal Mushroom from Taiwan Taiwanofungus salmoneus (Higher Basidiomycetes) Mycelium Combined with or without Cisplatin on Hepatocellular Carcinoma Cells
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Yun-Jung Hsieh, Jeng-Leun Mau, Rao-Chi Chien
Antiproliferative and Apoptotic Activities of the Medicinal Mushroom Phellinus rimosus (Agaricomycetes) on HCT116 Human Colorectal Carcinoma Cells
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 10
Chakrappilly Radhakrishnan Meera, Kainoor K. Janardhanan, Devarajan Karunagaran
Luteolin Induces Cell Cycle Arrest and Apoptosis Through Extrinsic and Intrinsic Signaling Pathways in MCF-7 Breast Cancer Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 3
Su-Ho Park, Jeoung-Woo Kang, Sunyoung Ham, Sei-Ryang Oh, Tae Ho Kwon, Do-Young Yoon, Man Sub Kim, Dong Hun Lee
Effect of Ukrain on Cell Survival and Apoptosis in the Androgen-Independent Prostate Cancer Cell Line PC-3
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
Sivakamasundari Velayutham, Sharmila Govindaraj, Arunakaran Jagadeesan, Kavitha Venkatesh, Arunkumar Ramachandran, Elumalai Perumal, Senthilkumar Kalimuthu
Hepatoma Cell Growth Inhibition by Inducing Apoptosis with Polysaccharide Isolated from Turkey Tail Medicinal Mushroom, Trametes versicolor (L.: Fr.) Lloyd (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 3
Xinzhong Cai, Xin Zhou, Lifen Tian, Yan Pi, Shouyi Qiao, Juan Lin