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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v16.i6.40
pages 541-553

Scientific Validation of the Chinese Caterpillar Medicinal Mushroom, Ophiocordyceps sinensis (Ascomycetes) from India: Immunomodulatory and Antioxidant Activity

Richa Rathor
Defense Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, Delhi-110054
Kamla P. Mishra
Defense Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, Delhi-110054
Mamta Pal
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Amitabh
Defense Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, Delhi-110054
Praveen Vats
Defense Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, Delhi-110054
Vandana Kirar
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Prem Singh Negi
Defence Agricultural Research Laboratory, Pithoragarh, Uttarakhand, 262 501 India ; Defence Institute of Bio-Energy Research, Haldwani, India
Kshipra Misra
Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, India

ABSTRACT

In the present study, we aimed to elucidate the antioxidant property and anti-inflammatory activity of the aqueous extract of the Indian species of Ophiocordyceps sinensis (AECS), which demonstrates medicinal activity against numerous diseases. The chemical composition of AECS was quantified using a colorimeteric technique to determine the total phenolic and flavonoid contents. Antioxidant activity was determined by assays for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)diammonium salt (ABTS); 2,2-diphenyl-1-picryl-hydrazyl (DPPH); and ferric reducing antioxidant power (FRAP). Adenosine nucleoside and nitrogenous bases (adenine and uracil) were also quantified by high-performance thin layer chromatography (HPTLC). Furthermore, the aqueous extract was also analyzed for anti-inflammatory activity in vitro using THP1 cells. THP1 cells were treated with and without lipopolysaccharide (LPS) and AECS (at 25 µg/mL, 50 µg/mL and 100 µg/mL, respectively) for 24 h. After 24 h, supernatants were harvested and kept at −80°C until the cytokine assays were performed. Furthermore, nitric oxide (NO) content was also estimated in treated and untreated murine peritoneal macrophages using Griess reagent. AECS significantly suppressed LPS-induced release of TNF-α and IL-1β in THP1 cells and significantly suppressed NO release in macrophage cells without exerting any toxic effect. These results indicate the anti-inflammatory activity of AECS. Additionally, this extract also has an antioxidant property, as high contents of phenols and flavonoids are present in the extract with considerable reducing power. The results of this study clearly demonstrate the potent antioxidant property and anti-inflammatory activity of AECS. Therefore, consumption of AECS may be clinically useful to protect against inflammatory diseases.


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