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International Journal of Medicinal Mushrooms

Published 12 issues per year

ISSN Print: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Spore Germination and Breeding Pattern in Grifola frondosa (Dicks.:Fr.) S.F. Gray

Volume 7, Issue 3, 2005, 414 pages
DOI: 10.1615/IntJMedMushrooms.v7.i3.570
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ABSTRACT

Grifola frondosa (Meripiliaceae, Polyporales), commonly called Maitake, grows on hardwood trees in the northern temperate forests of the eastern United States, Canada, Europe, and Asia. It produces sporophores that are edible with high organoleptic properties. In addition to Maitake’s use as food, it is known to exhibit medicinal properties and is thus a fungus of high economic importance today.
The attempt to develop commercial strains from isolates of G. frondosa distributed in the US revealed the difficulties encountered at spore germination. Therefore, it became necessary to investigate various physicochemical factors that can affect spore germination of G. frondosa. Plant hormones, gibberellic acid (GA) and indole-3-acetic acid (IAA), temperature, pH, sonication, mushroom extract, and soil extract were tested in laboratory experiments. Temperature and pH showed significantly higher spore germination values compared to the control (2.8 spores per plate). In 30 °C temperature pretreatment, an average of 28.6 spores germinated at pH 7 resulted in an average germination of 32.2 spores per plate. Some of the concentrations of plant hormones showed germination values that were significantly higher than the control. The concentrations of plant hormones that were not significantly higher than the control were 1.0 mg/L GA, 0.01 mg/L IAA, and 0.05 mg/L IAA. The effect of sonication on spore germination was also significantly higher than the control in all the time periods tested. Soil extract treatment had a negative effect on spore germination as the values were significantly lower than the control. It was therefore concluded that pH and heat pretreatments were the most appropriate factors to stimulate spore germination in .
A preliminary study to determine the breeding pattern in G. frondosa was also conducted. Single spore isolates (ssi) were selected and examined for clamp connections, and 10 clamp free ssi were selected for use in the mating experiment. Cross mating was conducted by co-cultivating pairs of single spore isolates on 2% PDA plates and incubating for 14 days. All ssi were crossed in all possible combinations. Examination to determine formation or absence of clamp connection under ×100 and ×400 magnification conducted on all crosses was conducted, and the results analyzed for the breeding pattern. The results indicated that G. frondosa exhibits a tetra-polar breeding pattern. Among the 10 isolates used, the distribution of mating alles were 2 ssi A1B1, 2 ssi A2B2, 3 ssi A1B2, and 3 ssi A2B1. It was observed that clamp connection formation in G. frondosa is more than 70% and 50% less than what we normally observe in dikaryotic cultures of commercially cultivated species of Pleurotus and Lentinus.

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