Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v7.i4.10
pages 513-524

Anti-β-Glucan Antibody in Bovine Sera

Ken-Ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Chikaku Dogasaki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan
Tsunenori Iriki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan
Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011; Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Yu-ichiro Kurone
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

ABSTRACT

We recently detected anti-β-glucan (BG) antibody, which was highly reactive and specific to fungal β-1,3- and β-1,6-BG in sera from human volunteers and DBA/1 and DBA/2 mice. In this study, we examined whether the anti-BG antibody exists in bovine serum and compared the reactivity to various BG fractions from medicinal and edible mushrooms. In experiments using polysaccharide-coated ELISA plates, normal bovine serum showed higher levels of reactivity to the hot water extract of Agaricus brasiliensis, AgHWE. The relative activity is comparable to Candida solubilized cell wall BG, CSBG. On the other hand, the sera weakly reacted to grifolan (GRN) from Grifola frondosa and sonifilan (SPG) from Schizophyllum commune. Furthermore, in a competive ELISA, this antibody cross-reacted to the β-1,6-glucan segment of CSBG and AgHWE. Anti-BG antibody plays a role in the recognition of BG and induces biological activity in collaboration with other recognition molecules, such as the β-glucan receptor. These facts strongly suggest that cows show β-glucan-specific immune response, and β-glucan from mushrooms are recognized and induce immunopharmacological activity in cows.


Articles with similar content:

Analysis of the Titer and Reactivity of Antibody/ies Against Fungal Cell Wall β-Glucans in Human Sera
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Mayu Morita, Yoshiyuki Adachi, Noriko N. Miura, Ying Liu, Naohito Ohno, Ken-Ichi Ishibashi, Masuro Motoi
Macrophages Stimulated by Polysaccharide Purified from Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae) Enhance mRNA Expression of Th1 Cytokine Including IL-12 and 18
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 4
Nobuhide Fujitake, Sachiko Kawakami, Masashi Mizuno
Specificity of the Immunomodulating Activity of Sasa veitchii (Japanese Folk Medicine Kumazasa) to Fungal Polysaccharides
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Wataru Sato, Naohito Ohno, Ken-Ichi Ishibashi
Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi
Characterization of Blood (β-1,3-glucan and Anti-(β-glucan Antibody in Hemodialysis Patients Using Culinary-Medicinal Royal Sun Agaricus, Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 2
Yoshiyuki Adachi, Masaharu Yoshida, Noriko N. Miura, Noriko Yoshikawa, Naohito Ohno, Ken-Ichi Ishibashi, Iwao Nakabayashi