Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020033845
pages 161-170

Antioxidant and Genotoxic Effects of Aqueous and Methanol Extracts from Two Edible Mushrooms from Turkey in Human Peripheral Lymphocytes

Bugrahan Emsen
Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey
Busranur Guven
Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey
Yasin Uzun
Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey
Abdullah Kaya
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey; Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey

ABSTRACT

This study reported the genetic and oxidative effects of aqueous and methanol extracts from two edible mushrooms, Lepista nuda (Bull.) Cooke and Pleurotus ostreatus (Jacq.) P. Kummer, in cultured human lymphocytes. Chromosome aberration (CA) and micronucleus (MN) assays were used for genotoxic influences estimation. In addition, the changes of total antioxidant capacity (TAC) and total oxidative stress (TOS) in the cells were monitored. The fungal extracts at all applied concentrations did not indicate significant differences (p > 0.05) in CA and MN analyses. Furthermore, while the treatments with maximum concentration of aqueous extract of L. nuda statistically (p < 0.05) increased TAC especially, TOS levels in the cells were reduced by them in comparison with negative control. Based on TAC analysis, low IC50 values belonging to aqueous (5.43 mg/L) and methanol (10.88 mg/L) extracts of L. nuda were remarkable. Our data demonstrated that the extracts obtained from P. ostreatus and especially L. nuda can be a new resource for therapeutics with their nonmutagenic and antioxidant features.

REFERENCES

  1. Devenish RJ, Lai S-C. Autophagy and burkholderia. Immunol Cell Biol. 2015;93(1):18-24.

  2. Da Silva FP, MacHado MCC. Antimicrobial peptides: clinical relevance and therapeutic implications. Peptides. 2012;36(2):308-14.

  3. Lu LL, Suscovich TJ, Fortune SM, Alter G. Beyond binding: antibody effector functions in infectious diseases. Nat Rev Immunol. 2018;18(1):46-61.

  4. Didierlaurent A, Goulding J, Hussell T. The impact of successive infections on the lung microenvironment. Immunology. 2007;122(4):457-65.

  5. Hage CA, Mohammed KA, Antony VB. Pathogenesis of pleural infection. Respirology. 2004;9(1):12-15.

  6. van der Veen EL, Bensch F, Glaudemans AWJM, Lubde Hooge MN, de Vries EGE. Molecular imaging to enlighten cancer immunotherapies and underlying involved processes. Cancer Treat Rev. 2018;70:232-44.

  7. Wahid B, Ali A, Rafique S, Waqar M, Wasim M, Wahid K, Idrees M. An overview of cancer immunotherapeutic strategies. Immunotherapy. 2018;10(11):999-1010.

  8. Gruber F, Ornelas CM, Karner S, Narzt M-S, Nagelreiter IM, Gschwandtner M, Bochkov V, Tschachler E. Nrf2 deficiency causes lipid oxidation, inflammation, and matrix-protease expression in DHA-supplemented and UVA-irradiated skin fibro-blasts. Free Radic Biol Med. 2015;88:439-51.

  9. Sweeney B, Puri P, Reen DJ. Modulation of immune cell function by polyunsaturated fatty acids. Pediatr Surg Int. 2005;21(5):335-40.

  10. Calder PC, Jensen GL, Koletzko BV, Singer P, Wanten GJA. Lipid emulsions in parenteral nutrition of intensive care patients: current thinking and future directions. Intensive Care Med. 2010;36(5):735-49.

  11. Khaper N, Bryan S, Dhingra S, Singal R, Bajaj A, Pathak CM, Singal PK. Targeting the vicious inflammation-oxidative stress cycle for the management of heart failure. Antioxidants Redox Signal. 2010;13(7):1033-49.

  12. Emsen B, Togar B, Turkez H, Aslan A. Effects of two lichen acids isolated from Pseudevernia furfuracea (L.) Zopf in cultured human lymphocytes. Z Naturforsch C J Biosci. 2018;73(7-8):303-12.

  13. Emsen B, Dogan M. Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Sci Pol Cultus. 2018;17(1):23-33.

  14. Chang ST, Wasser SP. Current and future research trends in agricultural and biomedical applications of medicinal mushrooms and mushroom products (review). Int J Med Mushrooms. 2018;20(11):1034-48.

  15. Wasser SP. Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19(4):279-317.

  16. Sadi G, Kaya A, Yalcin HA, Emsen B, Kocabas A, Kartal DI, Altay A. Wild edible mushrooms from Turkey as possible anticancer agents on HepG2 cells together with their antioxidant and antimicrobial properties. Int J Med Mushrooms. 2016;18(1):83-95.

  17. Meera CR, Janardhanan KK, Karunagaran D. Antiproliferative and apoptotic activities of the medicinal mushroom Phellinus rimosus (Agaricomycetes) on HCT116 human colorectal carcinoma cells. Int J Med Mushrooms. 2018;20(10):935-45.

  18. Xu H De, Cho S-S, Lee S-Y, Oh D-S, Lim S-K, Park D-H. Immune-stimulating effects of Mycoleptodonoides aitchisonii (Agaricomycetes) water extract via TNF-a and IFN-y. Int J Med Mushrooms. 2017;19(9):809-15.

  19. Muszynska B, Grzywacz-Kisielewska A, Kala K, Gdula-Argasinska J. Anti-inflammatory properties of edible mushrooms: a review. Food Chem. 2018;243:373-81.

  20. Kashina S, Villavicencio LLF, Zaina S, Ordaz MB, Sabanero GB, Fujiyoshi VT, Lopez MS. Activity of extracts from submerged cultured mycelium of winter mushroom, Flammulina velutipes (Agaricomycetes), on the immune system in vitro. Int J Med Mushrooms. 2016;18(1):49-57.

  21. Zhang Y, Zhang M, Jiang Y, Li X, He Y, Zeng P, Guo Z, Chang Y, Luo H, Liu Y, Hao C, Wang H, Zhang G, Zhang L. Lentinan as an immunotherapeutic for treating lung cancer: a review of 12 years clinical studies in China. J Cancer Res Clin Oncol. 2018;144(11):2177-86.

  22. Chien R-C, Lin Y-C, Mau J-L. Apoptotic effect of Taiwanofungus salmoneus (Agaricomycetes) mycelia and solid-state fermented products on cancer cells. Int J Med Mushrooms. 2017;19(9):477-495.

  23. Veena RK, Ajith TA, Janardhanan KK. Lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), prevents doxorubicin-induced cardiotoxicity in rats. Int J Med Mushrooms. 2018;20(8):761-74.

  24. Desjardin DE, Wood MG, Stevens FA. California mushrooms: the comprehensive identification guide. London: Timber Press; 2014.

  25. Toledo CV, Barroetaven C, Fernandes A, Barros L, Ferreira ICFR. Chemical and antioxidant properties of wild edible mushrooms from native nothofagus spp. forest, Argentina. Molecules. 2016;21(9):1201-7.

  26. Uddin Pk M, Sayful Islam M, Pervin R, Dutta S, Talukder RI, Rahman M. Optimization of extraction of antioxidant polysaccharide from Pleurotus ostreatus (Jacq.) P. Kumm. and its cytotoxic activity against murine lymphoid cancer cell line. PLoS One. 2019;14(1):e0209371.

  27. Quinones Munoz TA, Navarrete NS, Acosta DFC, Gurrola EEC, Carbajal GRH, Santos EDCV. The effect of growth substrate and extraction solvent on biological activities of oyster culinary medicinal mushroom Pleurotus ostreatus (Agaricomycetes). Int J Med Mushrooms. 2018;20(10):989-1001.

  28. Breitenbach J, Kranzlin F. Fungi of Switzerland. Volume 4. Lucerne, Switzerland: Verlag Mykologia; 1995.

  29. Emsen B, Guven B. Activities of two edible macrofungi, Coprinus comatus and Leucoagaricus leucothites in human lymphocytes: cytogenetic and biochemical study. Plant Biosyst. 2019:1-8. doi: 10.1080/11263504.2019.1612479.

  30. Gratia S, Kay L, Potenza L, Seffouh A, Novel-Chate V, Schnebelen C, Sestili P, Schlattner U, Tokarska-Schlattner M. Inhibition of AMPK signalling by doxorubicin: at the crossroads of the cardiac responses to energetic, oxidative, and genotoxic stress. Cardiovasc Res. 2012;95(3):290-99.

  31. Brahmi D, Ayed Y, Hfaiedh M, Bouaziz C, Mansour HB, Zourgui L, Bacha H. Protective effect of cactus cladode extract against cisplatin induced oxidative stress, genotoxicity and apoptosis in balb/c mice: combination with phytochemical composition. BMC Complement Altern Med. 2012;12:111.

  32. Tohamy AA, Abdel AA, Shafaa MW, Mahmoud WS. Alleviation of genotoxic effects of cyclophosphamide using encapsulation into liposomes in the absence or presence of vitamin C. Gen Physiol Biophys. 2012;31(1):85-91.

  33. Ikekawa T. Beneficial effects of edible and medicinal mushrooms on human health care. Int J Med Mushrooms. 2001;3(4):291-98.

  34. Ikekawa T. Cancer risk reduction by intake of mushrooms and clinical studies on EEM. Int J Med Mushrooms. 2005;7(3):347.

  35. Pinto S, Barros L, Sousa MJ, Ferreira ICFR. Chemical characterization and antioxidant properties ofLepista nuda fruiting bodies and mycelia obtained by in vitro culture: effects of collection habitat and culture media. Food Res Int. 2013;51(2):496-502.

  36. Barros L, Venturini BA, Baptista P, Estevinho LM, Ferreira ICFR. Chemical composition and biological properties of Portuguese wild mushrooms: a comprehensive study. J Agric Food Chem. 2008;56(10):3856-62.

  37. Du J, Guo H-B, Li Q, Forsythe A, Chen X-H, Yu X-D. Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers. PLoS One. 2018;13(8):e0202761.

  38. Xu L, Wang Q, Wang G, Wu JY. Contents and antioxidant activities of polysaccharides in 14 wild mushroom species from the forest of Northeastern China. Int J Med Mushrooms. 2015;17(12):1161-70.

  39. Sharif S, Rashid S, Atta A, Irshad A, Riaz M, Shahid M, Mustafa G. Phenolics, tocopherols and fatty acid profiling of wild and commercial mushrooms from Pakistan. J Biol Regul Homeost Agents. 2018;32(4):863-67.

  40. Jaya BPD, Widiastuti EL, Nurcahyani E, Sutyarso. Taurine and oyster mushroom (Pleurotus ostreatus) prevents oxidative damage in liver of mice induced by paraquat. Biomed Pharmacol J. 2017;10(4):1993-2000.

  41. Sirag HM. Biochemical and hematological studies for the protective effect of oyster mushroom (Pleurotus ostreatus) against glycerol-induced acute renal failure in rats. J Biol Sci. 2009;9(7):746-52.

  42. Kylyc A, Yesilada E. Preliminary results on antigenotoxic effects of dried mycelia of two medicinal mushrooms in Drosophila melanogaster somatic mutation and recombination test. Int J Med Mushrooms. 2013;15(4):415-21.

  43. Taira K, Miyashita Y, Okamoto K, Arimoto S, Takahashi E, Negishi T. Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea. Mutat Res. 2005;586(2):115-23.

  44. Hsu J-H, Li I-C, Jiang Y-M, Chen C-C. Toxicological evaluation of Lepista nuda (Bull. ex Fr.) Cooke mycelium produced by an in vitro culture methodology. Int J Pharm Pharm Sci. 2015;7(10):363-70.

  45. Ebrahimi A, Atashi A, Soleimani M, Mashhadikhan M, Barahimi A, Maghari A. Anti-invasive and antiproliferative effects of Pleurotus ostreatus extract on acute leukemia cell lines. J Basic Clin Physiol Pharmacol. 2018;29(1):95-102.

  46. Elbatrawy EN, Ghonimy EA, Alassar MM, Wu F-S. Medicinal mushroom extracts possess differential antioxidant activity and cytotoxicity to cancer cells. Int J Med Mushrooms. 2015;17(5):471-79.


Articles with similar content:

Antioxidant Potential of White Oyster Culinary-Medicinal Mushroom, Pleurotus florida (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Santosh Singh Bhadoriya, Priya Pardhi, Alok Pal Jain, Nikhil Jain, Aditya Ganeshpurkar, Gopal Rai
Effects of Cooking and In Vitro Digestion on Antioxidant Properties and Cytotoxicity of the Culinary-Medicinal Mushroom Pleurotus ostreatoroseus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 3
Pedro Henrique Alves da Silva, Tatiane Brugnari, Fabíola Dorneles Inácio, Mariene Marques Nolli, Alex Graça Contato, Rosane Marina Peralta, Cristina Giatti Marques de Souza, Camila Gabriel Kato
Evaluation of Bioactivities, Phenolic and Metal Content of Ten Wild Edible Mushrooms from Western Black Sea Region of Turkey
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 10
Demet Kizil, Semiha Yenigun, Tevfik Ozen, Ibrahim Turkekul, Hasan Cesur
Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Maja Kozarski, Anita Klaus, Jovana Vunduk, Leonardus Johannes Lambertus Donatus Van Griensven, Predrag Petrovic, Miomir Nikšić, Zeljko Zizak
Comparison of the Composition and Antioxidant Activities of Phenolics from the Fruiting Bodies of Cultivated Asian Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 10
Peter Chi Keung Cheung, Shaoling Lin, Lai Tsz Ching, Xinxin Ke