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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i2.40
pages 121-130

In Vitro Antitumor Activity and Structure Characterization of Ethanol Extracts from Wild and Cultivated Chaga Medicinal Mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae)

Yong Sun
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Ting Yin
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Xian-Hui Chen
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Gong Zhang
University of Winnipeg, Manitoba, Canada
Rempel B. Curtis
Richardson Center for Functional Foods and Nutraceuticals, University of Manitoba, Manitoba, Canada
Zhan-Hui Lu
Department of Food Science and Engineering, China Agricultural University, Beijing
Ji-Hong Jiang
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, China

ABSTRACT

Inonotus obliquus (Pers.:Fr.) Pilat has been traditionally used as a folk remedy for treatment of cancers, cardiovascular disease and diabetes in Russia, Poland, and most of the Baltic countries, but natural reserves of this fungus have nearly been exhausted. This study was designed to investigate the artificial cultivation of I. obliquus and the antitumor activity of its tissues. The ethanol extract of cultivated sclerotium had the highest cell growth inhibitory rate (74.6%) as determined by an 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. 78% of the bags produced sclerotia and only 6.17 g/bag of sclerotium was obtained. Extracts of the cultivated fruiting body showed 44.2% inhibitory activity against tumor cells. However, the yield was as high as 18.24 g/bag, and 98% of the bags produced fruiting body. The results of gas chromatography-mass spectroscopy (GC-MS) showed that similar compounds were extracted from the wild and cultivated samples. The principal compounds observed were lanosterol, inotodiol, and ergosterol. Their percentages of the mass fraction were 86.1, 59.9, and 71.8% of the total, for the wild sclerotium, cultivated sclerotium, and cultivated fruiting body, respectively. Ergosterol was found to be much higher (27.32%) in cultivated fruiting body. We conclude that cultivated fruiting body of I. obliquus obtained by inoculation of the substrate with spawn mycelium of the fifth generation could serve as an ideal substitute for the wild I. obliquus.


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