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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i1.100
pages 79-86

Effect of the Carbon Source and Inoculum Preparation Method on Laccase and Manganese Peroxidase Production in Submerged Cultivation by the Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution, Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel; and Durmishidze Institute of Biochemistry and Biotechnology,0159 Tbilisi, Georgia
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia
Yitzhak Hadar
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel

ABSTRACT

The genus Ganoderma includes medicinal species that belong to the group of white rot fungi due to their ability to produce extracellular ligninolytic enzymes: laccase and manganese peroxidase (MnP). The aim of this study was to evaluate the significance of various carbon and inoculum preparation methods for oxidative enzyme production by the medicinal mushroom G. lucidum. Mushroom growth, as well as the production of laccase and MnP, significantly depends on the inoculum age and cultivation method. Synthetic medium experiments have shown that xylose ensured the highest laccase activity of G. lucidum 447 accumulating 116 U L−1 after 12 days of submerged mushroom cultivation. In all lignocellulosic substrate experiment variants, laccase activity reached maximum after 8 days of mushroom cultivation. Analogical regularities were detected when the MnP activity was measured in submerged fermentation of ethanol production residue by G. lucidum 447. A more interesting finding is the fact that mycelium grown on solid agar medium and used as inoculum not only provided a 3-fold increase in laccase yield in submerged fermentation of both lignocellulosic substrates but also stimulated MnP accumulation by G. lucidum 447.


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