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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018028980
pages 1107-1119

Cross-Flow Ultrafiltration Fractions of a Cold Aqueous Extract of the Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Exhibit Apoptosis in Tumor Cells

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Anna Nickisch-Hartfiel
Department of Chemistry, Niederrhein University of Applied Sciences, Krefeld, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany

ABSTRACT

Lentinus edodes (shiitake) ranks among the most well-known medicinal mushrooms worldwide. Immune-modulating effects of shiitake extracts have been widely demonstrated in animals and humans. Apart from providing highly purified compounds such as lentinan, crude mushroom extracts also show antitumor activities. The direct cytotoxicity of crude preparations still requires investigation. The focus of our study lies in the molecular weight cutoff distribution of β-glucans and proteins in a cold aqueous extract from L. edodes. We applied cross-flow ultrafiltration to obtain 6 fractions with different molecular sizes. β-glucan and protein contents were quantified. We were able to show that only small amounts of β-glucans were extracted and that protein content decreases with fraction size. The cytotoxic potential of the cold aqueous preparation was demonstrated by analyzing inhibition of the growth of non-small cell lung cancer and triple-negative breast cancer cells.


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