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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020034124
pages 379-388

Medicinal Properties of Clitocybe brunneocaperata (Agaricomycetes) from India

Sanjit Debnath
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India
Kishan Saha
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India
Panna Das
Microbiology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India
Ajay Krishna Saha
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India


The present research documented the first evidence of Clitocybe brunneocaperata from Tripura, Northeast India. The main purposes of this study were identification of the mushroom and qualitative toxicity screening along with antibacterial and antioxidant activities of its methanolic extracts. The species has been recognized on the basis of the morphometric and molecular studies. Toxicity test revealed its inedibility nature. Methanolic extracts of C. brunneocaperata showed potent antibacterial, free radical scavenging effect (65.42%), chelating effects on ferrous ion (65.93%), total phenolic content (0.028 mg GAE/g), and flavonoid content (0.013 mg CE/g). The lowest EC50 values of the free radical scavenging effect (5.94 mg/ml) and chelating effects on ferrous ion (0.072 mg/ml) were observed at 16 and 1.5 mg/ml concentrations, respectively, which implied stronger capability of free radical scavenging. Therefore, further research is also required to isolate and characterize the principal components of C. brunneocaperata.


  1. Fries E. Systema mycologicum. Vol. 1. Lunds: 1821. p. 520.

  2. George P, Hegde N. Muscarinic toxicity among family members after consumption of mushrooms. Toxicol Int. 2013;20:113-15.

  3. Puschner B. Veterinary toxicology: Basic and clinical principles. 3rd ed. New York: Elsevier; 2018. p. 955-966.

  4. Kirk PM, Cannon PF, Minter DW, Stalpers JA. Ainsworth and Bisby's dictionary of the fungi. 10th ed. Wallingford (UK): CAB International; 2008.

  5. Kour S, Kour H, Kumar S, Sharma YP. New records of Clitocybe species from Jammu and Kashmir, India. Indian J Forest. 2015;38(1):43-46.

  6. Lima ADL, Costa Fortes R, Carvalho Garbi Novaes MR, Percario S. Poisonous mushrooms: A review of the most common intoxications. Nutr Hosp. 2012;27(2):402-8.

  7. Nieminen P, Kirsi M, Mustonen AM. Suspected myotoxicity of edible wild mushrooms. Exp Biol Med. 2006;231:221-28.

  8. Badalyan SM, Kues U, Melikyan LR, Navarro-Gonzalez M. Medicinal properties of Coprinoid mushrooms (Basidiomycetes, Agaricales). Int J Med Mushrooms. 2005;7:378-380.

  9. Chang S, Miles GP. Mushrooms: Cultivation, nutritional value, medicinal effects and environmental impact. Boca Raton, FL: CRC Press; 2004. p. 436.

  10. Ikekawa T. Beneficial effects of edible and medicinal mushrooms on health care. Int J Med Mushrooms. 2001;3:291-298.

  11. Islam T, Ganesan K, Xu B. New insight into mycochemical profiles and antioxidant potential of edible and medicinal mushrooms: A review. Int J Med Mushrooms. 2019;21(3):237-51.

  12. Liu D, Coloe S, Baird R, Pedersen J. Application of PCR to the identification of dermatophyte fungi. J Med Microbiol. 2000;49:493-97.

  13. Debnath S, Das AR, Karmakar P, Debnath G, Das P, Saha AK. Checklist of mushroom diversity in West Tripura, North-East India. In: Sinha S, Sinha RK, editors. Trends in frontal areas of plant science research. Tripura (India): Narosa Publishing; 2017. p. 205-13.

  14. Cooper JA. New species and combinations of some New Zealand agarics belonging to Clitopilus, Lyophyllum, Gerhardtia, Clitocybe, Hydnangium, Mycena, Rhodocollybia and Gerronema. Mycosphere. 2014;5(2):263-88.

  15. Sambrook J, Fritsch EF, Maniatis TA. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989. p. 745.

  16. Boratyn GM, Camacho C, Cooper PS, Coulouris G, Fong A, Ma N, Madden TL, Matten WT, McGinnis SD, Merezhuk Y, Raytselis Y, Sayers EW, Tao T, Ye J, Zaretskaya I. BLAST: A more efficient report with usability improvements. Nucleic Acids Res. 2013;41:29-33.

  17. Svrcek M. The illustrated book of mushrooms. London: Caxton; 1998. p. 19.

  18. Block SS, Stevens RL, Barreto A, Murrill WA. Chemical identification of amanita toxin in Mushrooms. Science. 1955;121:505-6.

  19. Mau LJ, Huang PN, Hung SJ, Chen CC. Antioxidant property of methanolic extracts from two kinds of Antrodiacamphorata mycelia. Food Chem. 2004;86:25-31.

  20. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40(6):945-48.

  21. Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem. 1990;38:674-77.

  22. Swain T, Hillis WE. The phenolic constituents of Purnusdomestica. I. The quantitative analysis of phenolic constituents. J Sci Food Agric. 1959;10:63-68.

  23. Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir KS. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm. 2004;54:65-72.

  24. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-96.

  25. Debnath S, Majumdar K, Das P, Saha AK. New distribution record of five species of Xylaria from Tripura, Northeast India. Res Rev J Life Sci. 2018;8(1):1-10.

  26. Debnath S, Majumdar K, Das P, Saha AK. A new report of Xylaria schreuderiana [Van der Byl.] from Tripura, Northeast India. Indian For. 2018b;144(11):1114-15.

  27. Debnath S, Chakraborty K, Datta BK, Das P, Saha AK. Trichoglossum tetrasporum, newly recorded from India. Mycotaxon. 2019;134:119-24.

  28. Roy DA, Borthakur M, Saha AK, Joshi SR, Das P. Molecular characterization and antioxidant potential of three wild culinary-medicinal mushrooms from Tripura, Northeast India. Int J Med Mushrooms. 2017;19(1):55-63.

  29. Zosel A, Stanton M. Death following ingestion of Clitocybe species mushroom. J Clin Toxicol. 2016;6:308.

  30. Clark ED, Smith CS. Toxicological studies on the mushrooms Clitocybe illudens and Inocybe infida. Mycologia. 1913;5(4):224-32.

  31. Okawa S, Matsuda M, Sugawara M, Kitabayashi A, Kuroki J. Clinical characteristics of toxin in Japanese poisonous Clitocybe acromelalga mushroom. J Neurol Sci. 2013;333(1);e519-e536.

  32. Genest K, Hughes DW, Rice WB. Muscarine in Clitocybe species. J Pharm Sci. 1968;57(2):331-33.

  33. Cochran KW, Cochran MW. Clitocybe clavipes: Antabuse-like reaction to alcohol. Mycologia. 1978;70:1124-26.

  34. Hu SH, Chen KS, Liu MY, Cheung PCK, Wang JC, Chang SJ. Optimization of submerged cultivation conditions for production of big cup culinary-medicinal mushroom Clitocybe maxima (Agaricomycetes) biomass with significant antioxidative and antihyperlipidemic activities. Int J Med Mushrooms. 2017;19(7):641-51.

  35. Ersel FY, Cavas L. Enzyme-based scavengers and lipid peroxidation in some wild edible Agaricales s.l. mushrooms from Mugla (Turkey). Int J Med Mushrooms. 2008;10(3):269-77.

  36. Vaz JA, Heleno SA, Martins A, Almeida GM, Vasconcelos MH, Ferreira ICFR. Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines. Food Chem Toxicol. 2010;48(10):2881-84.

  37. Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR. Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chem. 2010;119:1443-50.

  38. Mercan N, Duru ME, TurkoGlu A, Gezer K, Kivrak I, TurkoGlu H. Antioxidant and antimicrobial properties of ethanolic extract from Lepista nuda (Bull.) Cooke. Ann Microbiol. 2006;56(4):339-44.

  39. Chen MH, WS Li, Lue YS, Chu CL, Pan IH, Ko CH, Chen DY, Lin CH, Lin SH, Chang CP, Lin CC. Clitocybe nuda activates dendritic cells and acts as a DNA vaccine adjuvant. Evid Based Complement Alternat Med. 2013:2013;761454.

  40. Pohleven J, Kos J, Sabotic J. Medicinal properties of the genus Clitocybe and of Lectins from clouded funnel cap mushroom, C. nebularis (Agaricomycetes). A review. Int J Med Mushrooms. 2016;18(11):965-75.

  41. Josiana AV, Heleno, AS, Martins A, Almeida MG, Vasconcelos MH, Ferreira ICFR. Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines. Food Chem Toxicol. 2010;48(10):2881-84.

  42. Solak MH, Kalmis E, Saglam H, Kalyoncu F. Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. And Rhizopogon roseolus (Cords) T.M. Fries collected from Turkey. Phytother Res. 2006;20:1085-87.

  43. Kim YS, Lee IK, Seok SJ, Yun BS. Chemical constituents of the fruiting bodies of Clitocybe nebularis and their antifungal activity. Mycobiology. 2008;36:110-13.

  44. Kalyoncu F, Oskay M. Antimicrobial activities of four wild mushroom species collected from Turkey. Proceedings of the Sixth International Conference on Mushroom Biology and Mushroom Products; 2008 Sep 29-Oct 3; Bonn, Germany. p. 31-35.

  45. Yamac M, Bilgili F. Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates. Pharmacol Biol. 2006;44:660-67.

  46. Zheng SY, Liu QH, Zhang GQ, Wang HX, Ng TB. Purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom Clitocybe sinopica. Acta Biochim Pol. 2010;57(1):43-48.

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