Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.625 Factor de Impacto de 5 años: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v28.i3.20
pages 209-221

Regulation of Heme Synthesis and Proteasomal Activity by Copper: Possible Implications for Wilson's Disease

Ravit Hait-Darshan
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Tania Babushkin
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Zvi Malik
The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Israel

SINOPSIS

Wilson's disease (Wd) is a genetic disorder resulting in Cu2+ accumulation, and is caused by mutations in the ATP7B gene, the copper transporter. In vivo studies show a correlation between Cu2+ accumulation and malfunction of the heme biosynthesis pathway. In this study, we describe multiple effects of Cu2+ accumulation on heme synthesis, which, in turn, affect proteasomal activity. Cu2+ toxicity was examined in two hepatocellular carcinoma cell lines, HepG2 and Hep3B, with Hep3B cells containing an integrated hepatitis B virus genome. Exposure of HepG2 and Hep3B cells to Cu2+ inhibited the enzymes PBGD and ALAD of the heme synthesis pathway and, in parallel, upregulated heme oxygenase-1 (HO-1). Proto-porphyrin IX (PpIX) and the heme pool were reduced as a result of these processes. PpIX synthesis was found to be lower in cells expressing the mutant ATP7B (P1134P), compared to those expressing the WT enzyme. Proteasomal activity was inhibited under Cu2+ treatment in HepG2 cells; however, Cu2+ induced marked proteosomal acceleration in Hep3B cells. Under these conditions, Ub-conjugated proteins were gradually accumulated, whereas treatment with bathocuproine disulfonic acid (BCS), a Cu2+ chelator, reversed this effect. In conclusion, our data suggest that copper downregulates the heme synthesis pathway in hepatocellular cells and further reduces it in the presence of mutated ATP7B.


Articles with similar content:

Accelerated Proteasomal Activity Induced by Pb2+, Ga3+, or Cu2+ Exposure Does Not Induce Degradation of αt-Synuclein
Journal of Environmental Pathology, Toxicology and Oncology, Vol.28, 2009, issue 1
Nurit Grunberg-Etkovitz, Almog Avital, Daniel Offen, Zvi Malik, Nirit Lev, Debby Ickowicz
The Role of the Lkb1/AMPK Pathway in Hematopoietic Stem Cells and Leukemia
Critical Reviews™ in Oncogenesis, Vol.19, 2014, issue 5
Daisuke Nakada, Yusuke Saito
In Silico Analysis of Lanostanoids Characterized in Ganoderma Mushrooms (Agaricomycetes) as Potential Ligands of the Vitamin D Receptor
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Joaquin Antonio Quiroz Carranza, Jorge Suarez-Medellin, Ángel Trigos, Abraham M. Vidal-Limon, Thuluz Meza-Menchaca
Low Doses of Heavy Metals Disrupt Normal Structure and Function of Rat Platelets
Journal of Environmental Pathology, Toxicology and Oncology, Vol.20, 2001, issue 1
Rakhi Bose, Siragam Vinaya Kumar, Shelley Bhattacharya
Phosphotyrosine Recognition by the Raf Kinase Inhibitor Protein
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 1
Philip C. Simister, Nicholas M. Burton, R. Leo Brady