Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v28.i3.40
pages 231-234

2Am-DNT Induces Cell Death and Apoptosis in Human Cells

Hirendranath Banerjee
Department of Biological and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, USA
Z. Hawkins
Departments of Biological Sciences and Pharmaceutical Science, Elizabeth City State University, Elizabeth City, NC 27909, USA
M. Yakubu
Departments of Biological Sciences and Pharmaceutical Science, Elizabeth City State University, Elizabeth City, NC 27909, USA
D. Smoot
Departments of Biological Sciences and Pharmaceutical Science, Elizabeth City State University, Elizabeth City, NC 27909, USA
M. Asktorab
Department of Medicine, Howard University Cancer Center, Washington, DC 20059, USA
S. K. Dutta
Department of Medicine, Howard University Cancer Center, Washington, DC 20059, USA

SINOPSIS

During microbial or mammalian cell metabolism, TNT (2,4,6-tinitrotoluene) is reduced to 2Am-DNT (2-amino-4,6-dinitrotoluene), 4Am-DNT, or 2,4-diamino-NT (2,4-diaminonitrotoluelne) depending on the specific organism. The metabolite 2Am-DNT is the most common of the TBT biotransformation pathways in bacterial and fungal species studied to date. in the mammalian liver cells, TNT is metabolized to 2Am-DNT by the P450 enzyme system. Apoptosis is rapidly emerging as a relevant endpoint for detecting low-dose toxin exposure. We report in this study that 2Am-DNT treatment of mammalian cells causes cell death by apoptosis. Cell death was assayed by the Trypan Blue method. Apoptotic changes, such as DNA break down, were detected in treated cells by the production of a dark-brown DAB (diaminobenzidine) signal using the Fragel™ Klenow DNA fragment detection system, by immunohistochemical techniques with fluorescence microscopy, and by using a microplate reader for a single-stranded DNA binding assay. All of these results showed that 2am-DNT is toxic to mammalian cells and induces apoptosis.


Articles with similar content:

Luteolin Induces Cell Cycle Arrest and Apoptosis Through Extrinsic and Intrinsic Signaling Pathways in MCF-7 Breast Cancer Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 3
Su-Ho Park, Jeoung-Woo Kang, Sunyoung Ham, Sei-Ryang Oh, Tae Ho Kwon, Do-Young Yoon, Man Sub Kim, Dong Hun Lee
Label-free optical detection of optogenetic activation of cells using phase-sensitive Fourier domain optical coherence tomography
Visualization, Image Processing and Computation in Biomedicine, Vol.1, 2012, issue 1
Niloy Choudhury, Zhaoqiang Zhang, Samar Mohanty, Ling Gu, Feng Zhao
Hepatocellular Carcinoma and Possible Chemical and Biological Causes: A Review
Journal of Environmental Pathology, Toxicology and Oncology, Vol.36, 2017, issue 2
Belmar Kocer-Gumusel, Ming-Wei Chao, Didem Oral, Pinar Erkekoglu
Hepatoma Cell Growth Inhibition by Inducing Apoptosis with Polysaccharide Isolated from Turkey Tail Medicinal Mushroom, Trametes versicolor (L.: Fr.) Lloyd (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 3
Xinzhong Cai, Xin Zhou, Lifen Tian, Yan Pi, Shouyi Qiao, Juan Lin
Nonthermal Plasma Increases Expression of Wound Healing Related Genes in a Keratinocyte Cell Line
Plasma Medicine, Vol.3, 2013, issue 1-2
Sander Bekeschus, Ulrike Lindequist, Sybille Hasse, Annemarie Barton, Lena Bundscherer, Kai Masur, Klaus-Dieter Weltmann, Kristian Wende, Anke Schmidt