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Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2014011303
pages 233-237

Silence of PTEN in Colorectal Cancer Cells Via siRNA Inhibits Cell Growth

Yu-ga Du
Department of Gastroenterology, 174 Hospital of PLA, Xiamen, Fujian Province, China
Qiu-tang Sang
Department of Gastroenterology, 174 Hospital of PLA, Xiamen, Fujian Province, China
Yian-yan Xin
Department of Gastroenterology, 174 Hospital of PLA, Xiamen, Fujian Province, China
Xun-fei Yi
Department of Laboratory Medicine, Fuzong Clinical College of the Second Military Medical University, Fuzhou, Fujian 350025 China

SINOPSIS

Colorectal cancer is one of the most commonly diagnosed cancers and is a leading cause of cancerrelated death worldwide. In this study, we aimed to examine the expression of PTEN in human colorectal cancer cell lines HCT-8 and to further investigate the functions of PTEN in colorectal cancer cells. Therefore, we established stably transfected HCT-8 cell lines expressing siRNA targeting the PTEN gene. Cell proliferation and cell migration of the siPTEN cells were characterized by the CCK-8 assay and the Transwell assay, respectively. Our results show that constitutive knockdown of the PTEN gene in siPTEN cells significantly promoted cell proliferation and migration. These results suggest that PTEN may play important roles in colorectal cancer cell proliferation and migration.