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Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2016016320
pages 223-236

miRSNPs of miR1274 and miR3202 Genes that Target MeCP2 and DNMT3b Are Associated with Lung Cancer Risk: A Study Conducted on MassARRAY Genotyping

Cansu Ozbayer
School of Health Sciences, Dumlupinar University, Kutahya, Turkey
Irfan Degirmenci
Department of Medical Biology, Eskisehir Osmangazi University, Eskisehir, Turkey
Derya Ustuner
Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey
Guntulu Ak
Eskisehir Osmangazi University, Medical Faculty, Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey
Faruk Saydam
Recep Tayyip Erdogan University, Medical Faculty, Department of Medical Biology and Genetics, Rize, Turkey
Ertugrul Colak
Department of Biostatistics, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey
Hasan Veysi Gunes
Department of Medical Biology, Eskisehir Osmangazi University, Eskisehir, Turkey
Muzaffer Metintas
Eskisehir Osmangazi University, Medical Faculty, Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey

SINOPSIS

Genetic variants of miRNAs that target DNMTs and MBDs involved in DNA methylation were scanned with current databases, and 35 miRSNPs in 22 miRNA genes were identified. The aim of the study was to determine the association between these variants of miRNA genes and lung cancer (LC). DNA samples were isolated from blood samples and genotyped using a Sequenom MassARRAY System. An association between the rs188912830 gene variant of miR3202 that targets the MeCP2 protein and LC was indicated in both subtypes. The presence of the C-allele in patients with LC and its subtypes was significantly lower, and the absence of the C-allele was determined to increase the risk of LC by 7,429-times compared to the presence (p=0,010). The rs318039 gene variant of miR1274 that targets DNMT3b was found to be associated with LC subtypes. When allele distributions were compared, the numbers of individuals with the C-allele were significantly lower in the NSCLC and SCLC groups. No significant associations were found for the rs72563729 variant of the miR200b gene that targets DNMT3a or for the rs145416750 variant of the miR513c gene that targets TRDMT1. The other 33 variants were found to be ancestral genotypes. Consequently, rs188912830 and rs318039 variations were associated with LC subtypes. Importantly, this study is the first to indicate the functional characterisation of miRSNPs of genes that target DNA methylation.