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Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i1.30
pages 21-31

Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F

P. Pratheeshkumar
Amala Cancer Research Centre, Amala Nagar, Thrissur-680555, Kerala State, India
Girija Kuttan
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State, India

SINOPSIS

The objective of this study was to assess the effect of oleanolic acid, a triterpene on inducing apoptosis in B16F-10 melanoma cells. Treatment of B16F-10 cells with nontoxic concentration of oleanolic acid showed the presence of apoptotic bodies and induced DNA fragmentation in a dose depended manner. Cell cycle analysis and TUNEL assay were also confirmed the observation. The apoptotic genes p53, Bax, Caspase-9 and caspase-3 were found upregulated in oleanolic acid treated cells, whereas the anti-apoptotic gene Bcl-2 was downregulated. Oleanolic acid treatment also showed a downregulation of Cyclin D1 expression and upregulated p21 and p27 gene expression in B16F-10 melanoma cells. Oleanolic acid inhibited the activation and nuclear translocation of transcription factors such as NF-κB, c-fos, ATF-2 and CREB-1, and also down regulated the production and expression of TNF-α, IL-1β, IL-6 and GM-CS. These results suggest that oleanolic acid induces apoptosis through activating p53 induced caspase-mediated pro-apoptotic pathway and suppression of NF-κB induced Bcl-2 mediated anti-apoptotic pathway.


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