Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.625 Factor de Impacto de 5 años: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v27.i3.60
pages 219-232

Histochemical, Ultrastructural, and Biochemical Evidences for Azadirachta indica−Induced Apoptosis in Benzo(a)pyrene−Induced Murine Forestomach Tumors

Subhash Chander Gangar
UNIVERSITY OF COLORADO DENVER
Ashwani Koul
Department of Biophysics, Basic Medical Sciences Block, Panjab University, Chandigarh, India

SINOPSIS

The present study reports aqueous Azadirachta indica leaf extract (AAILE)−mediated induction of apoptosis in a murine forestomach tumorigenesis model. Histochemistry-based quantification of apoptosis revealed enhanced apoptotic index in the forestomach tumors of mice receiving AAILE along with benzo(a)pyrene (B(a)P). Transmission electron microscopy confirmed the presence of classical morphological features of apoptosis including chromatin condensation/marginalization, nuclear fragmentation, and formation of apoptotic bodies. Scanning electron microscopy showed surface modifications on the transformed squamous epithelial cells and certain mitotic cells among them over the forestomach tumors of mice receiving only B(a)P. In tumors of the mice receiving AAILE along with B (a)P, such mitotic cells were found to be absent; however, certain cells showing shrinkage and blebbings (characteristics of apoptosis) were observed. DNA fragmentation was observed to increase exclusively in the tumors of mice that received AAILE along with B(a)P. Lipid peroxidation (LPO) levels decreased in forestomach tissues of mice in all the groups studied when compared to control counterparts. However, levels of LPO were found to increase in the tumorous tissue of mice that received AAILE along with B(a)P when compared to mice receiving only B(a)P. Taken together, observations of the present study suggest that A. indica induces apoptosis in B(a)P-induced murine forestomach tumors.


Articles with similar content:

Curcumin Augments the Efficacy of Antitumor Drugs Used in Leukemia by Modulation of Heat Shock Proteins Via HDAC6
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 3
Apurba Mukherjee, Raj Biswas, Madhumita Roy, Sutapa Mukherjee, Jaydip Biswas, Ruma Sarkar
Curative Effect of Amorphophallus campanulatus (Roxb.) Blume. Tuber On N-Nitrosodiethylamine- Induced Hepatocellular Carcinoma in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 3
Santhibhavan Prabhakaran Prabha, Anand Nitha, Puthuparampil Nazarudeen Ansil, Mukalel Sankunni Latha
Antitumor Activity of Oral Administration of Mycovirus Extract from Lentinus edodes (Berk.) Sing. (Agaricomycetideae) on Murine Lymphoma
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 2
Mah-Lee Ng, Sudhir C. Kumar
Effect of Amentoflavone, a Phenolic Component From Biophytum sensitivum, on Cell Cycling and Apoptosis of B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 4
K.S. Siveen , Girija Kuttan
Protective Effects of Picorrhiza Kurroa Extract against 2-Acetylaminofluorene-Induced Hepatotoxicity in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 3
Sarwat Sultana, Sahar Rahman