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Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v25.i1-2.210
pages 321-340

Methods for Detailed Histopathological Investigation and Localization of Biopsies from Cervix Uteri to Improve the Interpretation of Autofluorescence Data

Sara Palsson
Lund University Medical Laser Centre; Department of Physics, Lund Institute of Technology, SE-221 00 Lund, Sweden; Science and Technology International, Honolulu, HI, 96813
Unne Stenram
Lund University Medical Laser Centre; Department of Pathology, Lund University Hospital, SE-221 85 Lund, Sweden
Marcelo Soto Thompson
Lund University Medical Laser Centre; Department of Physics, Lund Institute of Technology, SE-221 00 Lund, Sweden
Aurelija Vaitkuviene
Department of Obstetrics and Gynaecology, Vilnius University Hospital; Institute of Materials Science and Applied Research, Vilnius University, 2040 Vilnius, Lithuania
Violeta Poskiene
Department of Obstetrics and Gynaecology, Vilnius University Hospital, 2040-LT, Vilnius, Lithuania
Reda Ziobakiene
Department of Obstetrics and Gynaecology, Vilnius University Hospital, 2040-LT, Vilnius, Lithuania
Jody Oyama
Science and Technology International, Honolulu, HI, 96813
Michael J. DeWeert
Science and Technology International, Honolulu, HI, 96813
Ulf Gustafsson
Science and Technology International, Honolulu, HI, 96813
Niels Bendsoe
Department of Dermatology, Lund University Hospital, Lund University Medical Laser Centre, SE-221 00 Lund, Sweden; Department of Dermatology, Lund University Hospital, SE-221 85 Lund, Sweden
Stefan Andersson-Engels
Lund University Medical Laser Centre; Department of Physics, Lund Institute of Technology, SE-221 00 Lund, Sweden
Sune Svanberg
Lund University Medical Laser Centre; Department of Physics, Lund Institute of Technology, SE-221 00 Lund, Sweden
Katarina Svanberg
Lund University Medical Laser Centre; Department of Oncology, Lund University Hospital, SE-221 85 Lund, Sweden

SINOPSIS

Fluorescence spectroscopy is one of many optical methods that are potentially clinically useful for noninvasive detection and characterization of disorders on the cervical part of uterus, including precancerous lesions. The cervix uteri exhibits a biologically complex tissue and the morphology of a biopsy is generally not homogenous. The standard histopathological protocol accounts only for the most severe condition found within the biopsy and no information is given on other constituents potentially influencing the recorded fluorescence spectra. Spectra are usually correlated, using multivariate techniques, to the histopathological diagnosis of the biopsies. Since the probe volume of fluorescence spectroscopy is considerably smaller than the extension of the biopsy, this can cause problems in the search for correlation between the fluorescence signals and the pathological structures. In addition, the orientation and location of the biopsies are normally not recorded. We now report on the first detailed histopathological protocol where numerous tissue parameters, such as thickness and type of the epithelium and the number of blood vessels, glands, and inflammatory cells, are tabulated and the orientation and location of the biopsy are recorded as precisely as possible. Hopefully, the use of this protocol together with sophisticated mathematical methods will increase the probability to classify cervical disorders of the uterus, including precancerous lesions, with high sensitivity and specificity.


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