Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Journal of Environmental Pathology, Toxicology and Oncology
Factor de Impacto: 1.241 Factor de Impacto de 5 años: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i3.50
12 pages

Prevention and Repair of DNA Damage by Selected Phytochemicals as Measured by Single Cell Gel Electrophoresis

Sutapa Chakraborty
Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, Kolkata, India
Madhumita Roy
Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, Calcutta, India
Rathindra Kumar Bhattacharya
Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026

SINOPSIS

We assessed the ability of some natural products−namely, curcumin, resveratrol, indole-3-carbinol, and ellagic acid−to modify the DNA damaging ability of the alkylating carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in cultured Chinese hamster lung fibroblast cells (CH V-79). MNNG produced DNA single strand breaks in a dose- and time-dependent manner, as observed by increase in the tail moments of the comet, when the cells were subjected to alkaline single cell gel electrophoresis. When the cells were treated in the presence of each of the natural compounds, the DNA damage caused by MNNG was considerably reduced. This effect was found to be dose related. Preincubation of cells with each of these compounds individually afforded significant protection to DNA against damage caused by subsequent treatment with MNNG, indicating a true chemopreventive role of these substances. The most remarkable aspect of the present study was that all four compounds helped in the recovery of DNA damage by accelerating DNA repair efficiency in the damaged cells. This was further substantiated by the observation on unscheduled DNA synthesis. Our results suggest that these agents are chemopreventive by virtue of their ability to protect DNA as well as to induce DNA repair.


Articles with similar content:

Black Tea Protects Thymocytes in Tumor-Bearing Animals by Differential Regulation of Intracellular ROS in Tumor Cells and Thymocytes
Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 2
Sreya Chattopadhyay, Tanya Das, Arindam Bhattacharyya, Maqsood Siddiqi, Gaurisankar Sa, Lakshmishri Lahiry, Deba Prasad Mandal
Arsenic-Induced Micronuclei Formation in Mammalian Cells and Its Counteraction by Tea
Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 1
Maqsood Siddiqi, Madhumita Roy, Rathindra Kumar Bhattacharya, Dona Sinha
Effect of Amentoflavone, a Phenolic Component From Biophytum sensitivum, on Cell Cycling and Apoptosis of B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 4
K.S. Siveen , Girija Kuttan
Black Tea-Induced Amelioration of Hepatic Oxidative Stress through Antioxidative Activity in EAC-Bearing Mice
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 4
Sreya Chattopadhyay, Tanya Das, Arindam Bhattacharyya, Gaurisankar Sa, Lakshmishri Lahiry, Uttam K. Ghosh, Sankar Bhattacharyya, Deba Prasad Mandal
Biochanin A Enhances the Radiotoxicity in Colon Tumor Cells In Vitro
Journal of Environmental Pathology, Toxicology and Oncology, Vol.32, 2013, issue 3
Reeta Tiwari, Abhay Puthli, Kaushala Prasad Mishra