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Development and Application of Tissue Microarrays for Biomarker Studies

Volumen 2, Edición 2, 2011, pp. 145-159
DOI: 10.1615/ForumImmunDisTher.v2.i2.60
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SINOPSIS

Traditional methods for analyzing large numbers of tissue samples require significant amounts of time, plentiful costly reagents, and highly skilled technicians. The development of tissue microarrays has provided an alternative method, allowing for high-throughput screening and analysis of hundreds of samples on a single slide. This has powerful implications for the identification and characterization of biomarkers involved in cancer progression and outcome. In this review, we describe methods of designing and constructing a tissue microarray, as well as techniques for quantifying and analyzing biomarker expression using a tissue microarray.

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