Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Critical Reviews™ in Immunology
Factor de Impacto: 1.352 Factor de Impacto de 5 años: 3.347 SJR: 0.657 SNIP: 0.55 CiteScore™: 2.19

ISSN Imprimir: 1040-8401
ISSN En Línea: 2162-6472

Volumes:
Volumen 39, 2019 Volumen 38, 2018 Volumen 37, 2017 Volumen 36, 2016 Volumen 35, 2015 Volumen 34, 2014 Volumen 33, 2013 Volumen 32, 2012 Volumen 31, 2011 Volumen 30, 2010 Volumen 29, 2009 Volumen 28, 2008 Volumen 27, 2007 Volumen 26, 2006 Volumen 25, 2005 Volumen 24, 2004 Volumen 23, 2003 Volumen 22, 2002 Volumen 21, 2001 Volumen 20, 2000 Volumen 19, 1999 Volumen 18, 1998 Volumen 17, 1997 Volumen 16, 1996 Volumen 15, 1995 Volumen 14, 1994

Critical Reviews™ in Immunology

DOI: 10.1615/CritRevImmunol.2017018316
pages 359-378

Flow Cytometric Characterization of Antigen-Specific T Cells Based on RNA and Its Advantages in Detecting Infections and Immunological Disorders

Felix Radford
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
Sanjay Tyagi
Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, NJ 07103
Maria Laura Gennaro
Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, NJ 07103
Richard Pine
Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, NJ 07103
Yuri Bushkin
Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, NJ 07103

SINOPSIS

Fluorescence in situ hybridization coupled with flow cytometry (FISH-Flow) is a highly quantitative, high-throughput platform allowing precise quantification of total mRNA transcripts in single cells. In undiagnosed infections posing a significant health burden worldwide, such as latent tuberculosis or asymptomatic recurrent malaria, an important challenge is to develop accurate diagnostic tools. Antigen-specific T cells create a persistent memory to pathogens, making them useful for diagnosis of infection. Stimulation of memory response initiates T-cell transitions between functional states. Numerous studies have shown that changes in protein levels lag real-time T-cell transitions. However, analysis at the single-cell transcriptional level can determine the differences. FISH-Flow is a powerful tool with which to study the functional states of T-cell subsets and to identify the gene expression profiles of antigen-specific T cells during disease progression. Advances in instrumentation, fluorophores, and FISH methodologies will broaden and deepen the use of FISH-Flow, changing the immunological field by allowing determination of functional immune signatures at the mRNA level and the development of new diagnostic tools.


Articles with similar content:

Molecular Epidemiology of Human Cancer: Biomarkers of Genotoxic Exposure and Susceptibility
Journal of Environmental Pathology, Toxicology and Oncology, Vol.20, 2001, issue 4
Stephen G. Grant
Methylation-Based Biomarkers for Early Detection of Urological Cancer
Critical Reviews™ in Oncogenesis, Vol.13, 2007, issue 4
Carmen Jeronimo, Rui Manuel Ferreira Henrique, Vera L. Costa
Measuring T Cell Responses by Flow Cytometry–Based Fluorescence In Situ Hybridization
Critical Reviews™ in Immunology, Vol.38, 2018, issue 2
J.J. Freen-van Heeren , Benoit P. Nicolet, Monika C. Wolkers
Toward Prediction of Immune Mechanisms and Design of Immunotherapies in Melanoma
Critical Reviews™ in Biomedical Engineering, Vol.40, 2012, issue 4
Frank O. Nestle, Louise Saul, Sophia N. Karagiannis, Sophia Tsoka, Debra H. Josephs, Chrysanthi Ainali, Panagiotis Karagiannis
Natural Products as Inhibitors of Epidermal Growth Factor Receptor
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 4
Thomas Efferth