Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Plasma Medicine
SJR: 0.198 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimir: 1947-5764
ISSN En Línea: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2018027473
pages 39-48

Transient Spark for Bacterial Cleaning of Dental Microcavities

Loïc Ledernez
Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Albert-Ludwigs-University of Freiburg, Germany
R. Bruch
Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Albert-Ludwigs-University of Freiburg, Germany
M. Altenburger
Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg; Faculty of Medicine, University of Freiburg, Germany
M. Bergmann
Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Albert-Ludwigs-University of Freiburg, Germany
G. Urban
Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Albert-Ludwigs-University of Freiburg, Germany


We present a method to disinfect microcavities such as tooth root canals. With the aim of being introduced inside of the root canal, parameters are chosen to produce reactive oxygen species that inactivate bacteria and cause little to no temperature increase within the canal. We demonstrate the device's effects on bacteria with the use of Escherichia coli spread on agar in a Petri dish.


  1. Laroussi M. Sterilization of contaminated matter with an atmospheric pressure plasma. IEEE Trans Plasma Sci. 1996;24(3):1188-91.

  2. Stoffels E, FlikweertAJ, Stoffels WW, Kroesen GMW. Plasma needle: A non-destructive atmospheric plasma source for fine surface treatment of (bio)materials. Plasma Sources Sci Technol [Internet], 2002;11(4):383-8. Available from:

  3. Tendero C, Tixier C, Tristant P, Desmaison J, Leprince P. Atmospheric pressure plasmas: A review. Spectrochim Acta B Atom Spectrosc. 2006;61(1):2-30.

  4. Bogle MA, Arndt KA, Dover JS. Evaluation of plasma skin regeneration technology in low-energy full-facial rejuvenation. Arch Dermatol [Internet]. 2007;143(2):168-74. Available from:

  5. Heinlin J, Morfill G, Landthaler M, Stolz W, Isbary G, Zimmermann JL, Shimizu T, Karrer S. Plasma medicine: Possible applications in dermatology. J Dtsch Dermatol Ges. 2010 Dec;8(12):968-76.

  6. von Woedtke T, Reuter S, Masur K, Weltmann KD. Plasmas for medicine. Phys Rep. 2013;530(4):291-320. Available from:

  7. Robert E, Barbosa E, Dozias S, Vandamme M, Cachoncinlle C, Viladrosa R, Pouvesle JM. Experimental study of a compact nanosecond plasma gun. Plasma Process Polym. 2009;6(12):795-802.

  8. Vandamme M, Robert E, Lerondel S, Sarron V, Ries D, Dozias S, Sobilo J, Gosset D, Kieda C, Legrain B, Pouvesle JM, Pape AL. ROS implication in a new antitumor strategy based on nonthermal plasma. Int J Cancer. 2012;130(9):2185-94.

  9. Georgescu N, Lupu AR. Tumoral and normal cells treatment with high-voltage pulsed cold atmospheric plasma jets. IEEE Trans Plasma Sci. 2010;38(8 Part 2):1949-55.

  10. Emmert S, Brehmer F, HanBle H, Helmke A, Mertens N, Ahmed R, Simon D, Wandke D, Maus-Friedrichs W, Daschlein G, Schon MP, Viol W. Atmospheric pressure plasma in dermatology: Ulcus treatment and much more. Clin Plasma Med [Internet], 2013 Jun;l(l):24-9. Available from: http:// linkinghub.elsevier. com/retrieve/pii/S2212816612000042.

  11. Jiang C, Chen MT, GorurA, Schaudinn C, Jaramillo DE, Costerton JW, Sedghizadeh PP, Vernier PT, Gundersen MA. Nanosecond pulsed plasma dental probe. Plasma Process Polym. 2009;6(8):479-83.

  12. Taschieri S, Del Fabbro M, Samaranayake L, Chang JWW, Corbella S. Microbial invasion of dentinal tubules: A literature review and a new perspective. JInvestig Clin Dent [Internet], 2014;5(3):163-70. Available from:

  13. Kirkevang LL, VsthM, Wenzel A. Ten-year follow-up of root filled teeth: A radiographic study of a Danish population. Int Endod J. 2014;47(10):980-8.

  14. Moghaddam AS, Radafshar G, Taramsari M, Darabi F. Long-term survival rate of teeth receiving multi-disciplinary endodontic, periodontal and prosthodontic treatments. J Oral Rehabil. 2014;41(3):236-42.

  15. Mehdipour O, Kleier DJ, Averbach RE. Anatomy of sodium hypochlorite accidents. Compend Con-tin Educ Dent. 2007;28:544-6, 548, 550.

  16. Goswami M, Chhabra N, Kumar G, Verma M, Chhabra A. Sodium hypochlorite dental accidents. Paediatr Int Child Health [Internet], London, UK: Taylor & Francis; 2014. pp. 66-9. Available from:

  17. Laroussi M Leipold F. Evaluation of the roles of reactive species, heat, and UV radiation in the inactiva-tion of bacterial cells by air plasmas at atmospheric pressure. Int J Mass Spectrom. 2004;233(l-3):81-6.

  18. Rupf S, Lehmann A, Hannig M, Schafer B, Schubert A, Feldmann U, Schindler A. Killing of adherent oral microbes by a non-thermal atmospheric plasmajet. J Med Microbiol. 2010;59(2):206-12.

  19. Kanazawa S, Kawano H, Watanabe S, Furuki T, Akamine S, Ichiki R, Ohkubo T, Kocik M, Mizeraczyk J. Observation of OH radicals produced by pulsed discharges on the surface of a liquid. Plasma Sources Sci Technol. 2011;20(3):034010.

  20. Yamazaki H, Ohshima T, Tsubota Y, Yamaguchi H, Jayawardena JA, Nishimura Y. Microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens. Dent Mater J [Internet], 2011;30(3):384-91. Available from:

  21. Bettaieb A, Averill-Bates D. Thermotolerance induced at a mild temperature of 40 C protects cells against heat shock-induced apoptosis. J Cell Physiol. 2005 Oct;2Q5(l):47-57.

Articles with similar content:

Hydra as a Model for Screening Ecotoxicological Effects of Plasma-Treated Water
Plasma Medicine, Vol.8, 2018, issue 3
C. Sarangapani, D. Boehm, P. Bourke, Dana Ziuzina
Telomerase Inhibitory Effects of Medicinal Mushrooms and Lichens, and their Anticancer Activity
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 1
Baojun Xu, Chantian Li, Changkeun Sung
Antiproliferative Activity of Some Higher Mushrooms from Hungary against Human Cancer Cell Lines
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Attila Vanyolos, Istvan Zupko, Noemi Bozsity, Judit Hohmann, Bernadett Kovacs
Broccoli: Antimicrobial Efficacy and Influences to Sensory and Storage Properties by Microwave Plasma-Processed Air Treatment
Plasma Medicine, Vol.6, 2016, issue 3-4
Mathias Andrasch, Oliver Schlüter, Marion Jakobs, Kai-Uwe Katroschan, Uta Schnabel, Rijana Niquet, Klaus-Dieter Weltmann, Jörg Ehlbeck
Antiproliferative Activity and Cytotoxicity of Some Medicinal Wood-Destroying Mushrooms from Russia
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 1
Cesar Espinoza, Alla V. Shnyreva, Ángel Trigos, José M. Padrón, Anastasia A. Shnyreva