Publicado 6 números por año
ISSN Imprimir: 1045-4403
ISSN En Línea: 2162-6502
Indexed in
Functional Genomics in Rat Models of Hypertension: Using Differential Expression and Congenic Strains to Identify and Evaluate Candidate Genes
SINOPSIS
Hypertension is a leading contributor to cardiovascular diseases such as heart attack and stroke. Genetic and environmental factors contribute to the development of hypertension. Animal models have been developed to study the genetic contributions to blood pressure (BP) regulation and to identify chromosomal regions harboring candidate genes causative of differences in BP regulation (i.e., BP quantitative trait loci [QTL]). Advances in both mammalian genome projects and global gene expression analysis present opportunities to study functional genomics in these animal models. In this article, novel approaches for designing experiments and interpreting global gene expression data using the Dahl salt-sensitive hypertension rat model are presented. We describe two-step screening protocols that can be used to identify BP QTL candidate genes. Genetically determined expression differences are identified in the target organs of inbred strains of contrasting phenotype in the first screen. Expression patterns in a panel of congenic strains or expression differences stemming from gene x environment interactions are examined in the second screen. Chromosomal locations of these genes can then be examined to determine whether they map to BP QTL–containing regions. Another approach is to study the expression of genes identified from public databases to be located within BP QTL–containing congenic regions. Several candidate genes have been identified using these strategies.
-
Saavedra Juan M, Opportunities and limitations of genetic analysis of hypertensive rat strains, Journal of Hypertension, 27, 6, 2009. Crossref
-
Toland Edward J., Saad Yasser, Yerga-Woolwine Shane, Ummel Steven, Farms Phyllis, Ramdath Ramona, Frank Bryan C., Lee Norman H., Joe Bina, Closely linked non-additive blood pressure quantitative trait loci, Mammalian Genome, 19, 3, 2008. Crossref
-
Castaneda Carol, LaPointe Michael S., Gold Standard Animal Models, in Pharmaceutical Sciences Encyclopedia, 2011. Crossref
-
Saad Yasser, Garrett Michael R., Manickavasagam Ezhilarasi, Yerga-Woolwine Shane, Farms Phyllis, Radecki Tracy, Joe Bina, Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10, Genomics, 89, 3, 2007. Crossref
-
Ulfenborg Benjamin, Jurcevic Sanja, Lindlöf Angelica, Klinga-Levan Karin, Olsson Björn, miREC: a database of miRNAs involved in the development of endometrial cancer, BMC Research Notes, 8, 1, 2015. Crossref
-
Sommer Stacy L., Berndt Theresa J., Frank Elena, Patel Jeetendra B., Redfield Margaret M., Dong Xiangyang, Griffin Matthew D., Grande Joseph P., van Deursen Jan M. A., Sieck Gary C., Romero Juan C., Kumar Rajiv, Elevated blood pressure and cardiac hypertrophy after ablation of thegly96/IEX-1gene, Journal of Applied Physiology, 100, 2, 2006. Crossref
-
Lee Soon Jin, Ways Justin A., Barbato John C., Essig David, Pettee Krista, DeRaedt Sarah J., Yang Siming, Weaver David A., Koch Lauren G., Cicila George T., Gene expression profiling of the left ventricles in a rat model of intrinsic aerobic running capacity, Physiological Genomics, 23, 1, 2005. Crossref
-
NARABA Hiroaki, IWAI Naoharu, Assessment of the MicroRNA System in Salt-Sensitive Hypertension, Hypertension Research, 28, 10, 2005. Crossref
-
Garrett Michael R., Meng Haijin, Rapp John P., Joe Bina, Locating a Blood Pressure Quantitative Trait Locus Within 117 kb on the Rat Genome, Hypertension, 45, 3, 2005. Crossref